Thin layer and column chromatographic group separations of steroids as trimethylsilyl ethers. Isolation for GLC analysis of pregnanediol and estriol in pregnancy urine
| Autor: | C. J. W. Brooks, E.C. Horning, E.M. Chambaz |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
Chromatography
Gas Chemical Phenomena Trimethylsilyl medicine.medical_treatment Silicones Biophysics Urine Biochemistry Steroid chemistry.chemical_compound Pregnancy Group (periodic table) medicine Humans Organic chemistry Molecular Biology Chromatography Estriol Cell Biology medicine.disease 17-Ketosteroids Chemistry Models Chemical chemistry Androgens Pregnanediol Female Indicators and Reagents Steroids Chromatography Thin Layer Hydroxysteroids Androstanes Ethers |
| Zdroj: | Analytical Biochemistry. 19:234-242 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(67)90158-3 |
| Popis: | Trimethylsilyl ethers of steroids may be separated by thin-layer and column chromatographic methods. They are suitable derivatives for the group separation of hydroxysteroids from hydroxyketosteroids prior to gas chromatographic analysis. Group separations of two model steroid mixtures are described. An application of the method has been made to the simultaneous analytical separation of estriol and pregnanediol in human pregnancy urine; the determination of these two steroids is often of interest in pregnancy studies. |
| Databáze: | OpenAIRE |
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