Novel O-GlcNAcylation on Ser(40) of canonical H2A isoforms specific to viviparity
| Autor: | Kunio Shiota, Koji Hayakawa, Daisuke Arai, Takehiro Suzuki, Satoshi Tanaka, Mitsuko Hirosawa, Chikako Yoneda, Hitoshi Shiota, Naoshi Dohmae |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Gene isoform medicine.drug_class Xenopus Monoclonal antibody Genome Mass Spectrometry Article Acetylglucosamine Histones 03 medical and health sciences Mice Species Specificity Histone H2A medicine Serine Nucleosome Animals Humans Protein Isoforms Epigenetics Zebrafish Chromatography High Pressure Liquid Embryonic Stem Cells Phylogeny Multidisciplinary Binding Sites biology Antibodies Monoclonal biology.organism_classification Bombyx Molecular biology Biological Evolution Recombinant Proteins Cell biology Trophoblasts Mice Inbred C57BL 030104 developmental biology Macaca Cattle HeLa Cells |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | We report here newly discovered O-linked-N-acetylglucosamine (O-GlcNAc) modification of histone H2A at Ser40 (H2AS40Gc). The mouse genome contains 18 H2A isoforms, of which 13 have Ser40 and the other five have Ala40. The combination of production of monoclonal antibody and mass spectrometric analyses with reverse-phase (RP)-high performance liquid chromatography (HPLC) fractionation indicated that the O-GlcNAcylation is specific to the Ser40 isoforms. The H2AS40Gc site is in the L1 loop structure where two H2A molecules interact in the nucleosome. Targets of H2AS40Gc are distributed genome-wide and are dramatically changed during the process of differentiation in mouse trophoblast stem cells. In addition to the mouse, H2AS40Gc was also detected in humans, macaques and cows, whereas non-mammalian species possessing only the Ala40 isoforms, such as silkworms, zebrafish and Xenopus showed no signal. Genome database surveys revealed that Ser40 isoforms of H2A emerged in Marsupialia and persisted thereafter in mammals. We propose that the emergence of H2A Ser40 and its O-GlcNAcylation linked a genetic event to genome-wide epigenetic events that correlate with the evolution of placental animals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|