The lower matrix protein pp65 is the principal viral antigen present in peripheral blood leukocytes during an active cytomegalovirus infection
| Autor: | J. M. M. Grefte, B. T. F. van der Gun, S. Schmolke, M. van der Giessen, W. J. van Son, B. Plachter, G. Jahn, T. H. The |
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| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Human cytomegalovirus
EXPRESSION MONOCLONAL-ANTIBODY medicine.drug_class viruses Fluorescent Antibody Technique VIRUS INFECTIONS Enzyme-Linked Immunosorbent Assay VIREMIA Biology Antibodies Viral Monoclonal antibody Immediate early protein Immediate-Early Proteins Viral Matrix Proteins Antigen Antibody Specificity Virology INSITU HYBRIDIZATION Leukocytes medicine Viral structural protein Humans Antigens Viral Cell Nucleus Viral Structural Proteins Viral matrix protein TRANSPLANTATION Antibodies Monoclonal virus diseases LOCALIZATION Phosphoproteins medicine.disease Precipitin Tests KINASE ACTIVITY Recombinant Proteins Cytomegalovirus Infections Immunology Leukocytes Mononuclear biology.protein GENE-PRODUCTS Viral disease Antibody PP65 |
| Zdroj: | Journal of general virology, 73, 2923-2932. AMER SOC MICROBIOLOGY |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-73-11-2923 |
| Popis: | During an active infection with human cytomegalovirus (HCMV), viral antigen is consistently present in peripheral blood leukocytes. Two monoclonal antibodies (MAbs), CMV-C10 and CMV-C11, are commonly used in the HCMV antigenaemia assay to detect these cells in the peripheral blood of patients suspected of having an active HCMV infection. We demonstrate that the viral antigen detected by these MAbs is the viral structural protein pp65 and not an immediate early antigen as previously reported. Furthermore, significantly fewer leukocytes were found to be positive with MAbs specific for immediate early antigens. |
| Databáze: | OpenAIRE |
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