Viability and growth of mouse embryos after in vitro culture and fusion
| Autor: | Patricia Bowman, Anne McLaren |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Time Factors
Placenta Uterus Pronase Biology Andrology Embryonic and Fetal Development Mice Pregnancy Culture Techniques medicine Animals Embryo Implantation Blastocyst Zona pellucida Molecular Biology Incubation reproductive and urinary physiology Analysis of Variance Fetus urogenital system Embryo Organ Size Anatomy Culture Media medicine.anatomical_structure embryonic structures Female Developmental Biology |
| Zdroj: | Development. 23:693-704 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.23.3.693 |
| Popis: | About 80 % of 8-cell mouse eggs developed to the blastocyst stage in culture, whether the zona pellucida was left intact, or removed with pronase (pre-incubated and dialysed) and the eggs then cultured singly or as fused pairs. When pronase was used without prior incubation and dialysis, the success rate was reduced to 50 %. After transfer to uterine foster-mothers, 20–30 % of apparently normal blastocysts cultured with or without the zona, singly or fused, developed into live foetuses, compared with over 50 % of control blastocysts taken directly from the uterus. Some of the excess mortality of cultured embryos took place before implantation and some soon after. The foetuses derived from cultured blastocysts averaged 0·1g lighter than those derived from control uterine blastocysts similarly transferred. No differences in the weights of the placentae were observed. Foetal and placental weights were unaffected by whether the eggs had been cultured singly or fused, implying that growth regulation of fused embryos is complete by the 17th day of gestation. The longer the eggs were maintained in culture, the lower was their viability after transfer, and the lighter were the foetuses derived from them. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|