Molecular cloning and ethylene-inducible expression of Chib1 chitinase from soybean (Glycine max (L.) Merr.)
| Autor: | Akira Watanabe, Nana Asare Yeboah, Kyoko Udaka, Deyu Zhang, Chikafusa Fukazawa, Van Hai Nong, Masaomi Arahira |
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| Rok vydání: | 1999 |
| Předmět: |
DNA
Plant Sequence analysis Molecular Sequence Data Sequence alignment Biology Molecular cloning Applied Microbiology and Biotechnology Biochemistry Polymerase Chain Reaction Analytical Chemistry Plant Growth Regulators Gene Expression Regulation Plant Amino Acid Sequence Cloning Molecular Molecular Biology Peptide sequence Phylogeny DNA Primers Plant Proteins chemistry.chemical_classification Molecular mass Base Sequence Sequence Homology Amino Acid Organic Chemistry Chitinases Nucleic acid sequence food and beverages General Medicine Sequence Analysis DNA Ethylenes Blotting Northern Molecular biology Amino acid Molecular Weight Blotting Southern chemistry RNA Plant Chitinase biology.protein Soybeans Sequence Alignment Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 63(2) |
| ISSN: | 0916-8451 |
| Popis: | A soybean seed-specific PR-8 chitinase, named Chib2, has a markedly extended C-terminal segment compared to other plant Chib1 homologues of the PR-8 chitinase family known to date. To further characterize the molecular structure and the expression pattern of this chitinase family, we cloned two typical Chib1-similar cDNAs (Chib1-1 and Chib1-2) from soybeans by PCR-cloning techniques. The deduced primary sequence of Chib1-1 chitinase is composed of a signal peptide segment (26 amino acid residues) and a mature 273 amino acid sequence (calculated molecular mass 28,794, calculated pI 3.7). This Chib1-1 enzyme is more than 90% identical to Chib1-2 chitinase but is below 50% identical to Chib2 enzyme. Thus, we confirmed the occurrence of two distinct classes, Chib1 and Chib2 in the plant PR-8 chitinase family. The Chib1 genes, interrupted by one intron, were found to be up-regulated in response to ethylene in stems and leaves, but scarcely expressed in developing soybean seeds. Chib1 chitinases may be responsible for protecting the plant body from various pathogenic attacks. |
| Databáze: | OpenAIRE |
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