Embryonic Steroids Control Developmental Programming of Energy Balance
Autor: | Meng-Chun Monica Shih, Chen-Che Jeff Huang, Bon Chu Chung, Nai-Chi Hsu, Hsueh-Ping Chu |
---|---|
Rok vydání: | 2021 |
Předmět: |
Male
medicine.medical_specialty Mice 129 Strain medicine.medical_treatment Embryonic Development Mice Transgenic Energy homeostasis Dexamethasone chemistry.chemical_compound Mice Endocrinology Pregnancy Internal medicine medicine Animals Cholesterol Side-Chain Cleavage Enzyme Glycogen synthase Fetus biology Glycogen Cholesterol side-chain cleavage enzyme Insulin Gluconeogenesis Embryo Mammalian Mice Inbred C57BL chemistry Animals Newborn Prenatal Exposure Delayed Effects biology.protein Female Steroids Energy source Energy Metabolism Glucocorticoid medicine.drug |
Zdroj: | Endocrinology. 162(12) |
ISSN: | 1945-7170 |
Popis: | Glucose is a major energy source for growth. At birth, neonates must change their energy source from maternal supply to its own glucose production. The mechanism of this transition has not been clearly elucidated. To evaluate the possible roles of steroids in this transition, here we examine the defects associated with energy production of a mouse line that cannot synthesize steroids de novo due to the disruption of its Cyp11a1 (cytochrome P450 family 11 subfamily A member 1) gene. The Cyp11a1 null embryos had insufficient blood insulin and failed to store glycogen in the liver since embryonic day 16.5. Their blood glucose dropped soon after maternal deprivation, and the expression of hepatic gluconeogenic and glycogenic genes were reduced. Insulin was synthesized in the mutant fetal pancreas but failed to be secreted. Maternal glucocorticoid supply rescued the amounts of blood glucose, insulin, and liver glycogen in the fetus but did not restore expression of genes for glycogen synthesis, indicating the requirement of de novo glucocorticoid synthesis for glycogen storage. Thus, our investigation of Cyp11a1 null embryos reveals that the energy homeostasis is established before birth, and fetal steroids are required for the regulation of glycogen synthesis, hepatic gluconeogenesis, and insulin secretion at the fetal stage. |
Databáze: | OpenAIRE |
Externí odkaz: |