The Herbal Medicine Unkei-To Stimulates Cytokine-Induced Neutrophil Chemoattractant Production in the Pituitary Folliculo-Stellate-Like Cell Line (TtT/GF)
| Autor: | Hiroaki Jikihara, Koji Koike, Kanji Masuhara, Masaki Inoue, Zhen X. Zhang, Kouichi Murakami, Yoshiki Sakamoto, Akira Miyake |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Pituitary gland medicine.medical_treatment education Immunology Follicular cell Cell Line Anterior pituitary Pituitary Gland Anterior Internal medicine medicine Animals Immunology and Allergy Secretion RNA Messenger Northern blot Rats Wistar Growth Substances Medicine East Asian Traditional Plants Medicinal Chemotactic Factors Dose-Response Relationship Drug business.industry Obstetrics and Gynecology Drug Synergism Rats Cytokine medicine.anatomical_structure Endocrinology Reproductive Medicine Cell culture Cytokines Intercellular Signaling Peptides and Proteins Female business Chemokines CXC Drugs Chinese Herbal Endocrine gland |
| Zdroj: | American Journal of Reproductive Immunology. 39:249-255 |
| ISSN: | 1046-7408 |
| DOI: | 10.1111/j.1600-0897.1998.tb00361.x |
| Popis: | The herbal medicine unkei-to stimulates cytokine-induced neutrophil chemoattractant production in the pituitaryfolliculo-stellate-like cell line (TtT/GF) PROBLEM: We previously reported that a cytokine-induced neutrophil chemoattractant (CINC) was produced in the pituitary gland and that it influenced anterior pituitary hormone release. In this study we investigated the effect of Unkei-to, a Japanese herbal medicine, on CINC production in the rat anterior pituitary gland and the pituitary folliculo-stellate-like cell line (TtT/GF). METHOD OF STUDY: Dispersed normal anterior pituitary cells and the folliculo-stellate-like cell line TtT/GF were used to test the effect of Unkei-to on CINC secretion and CINC mRNA accumulation. Concentrations of CINC in the conditioned media were measured by an enzyme-linked immunosorbent assay, and levels of CINC mRNA were analyzed by Northern blot analysis. RESULTS: Unkei-to (20 μg/ml) significantly increased the secretion of CINC by normal anterior pituitary cells within 12 hr of incubation. Unkei-to also stimulated CINC secretion from TtT/GF cells in a time- and dose-dependent manner. Unkei-to (20 μg/ml) increased CINC mRNA accumulation in TtT/GF cells within 3 hr of incubation and also caused a 13-fold increase in the secretion of CINC from TtT/GF cells compared with the vehicle group within 24 hr of incubation. Finally, we found that some of the Unkei-to's ingredients, Evodiae fructus and Pinelliae tuber, markedly stimulated CINC secretion from TtT/GF cells. CONCLUSIONS: Our results will help to elucidate the mechanism behind the clinical effect of Unkei-to on the anterior pituitary gland. They also suggested the presence of special substances, which stimulate CINC secretion, within Unkei-to's ingredients such as E. fructus and P. tuber. |
| Databáze: | OpenAIRE |
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