A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection
| Autor: | A. Khairul Anuar, S Lokman Hakim, N. Rahmah, R.K. Shenoy, P. Chotechuang, C.P. Ramachandran, Boon Huat Lim, V. Kumaraswami, K. Kanjanopas |
|---|---|
| Předmět: |
Recombinant antigen
Blotting Western Helminthiasis Enzyme-Linked Immunosorbent Assay Optical density Biology Sensitivity and Specificity Brugia malayi Filariasis Serology parasitic diseases medicine Animals Humans Public Health Environmental and Occupational Health General Medicine medicine.disease biology.organism_classification Virology Predictive value Recombinant Proteins Infectious Diseases Antigens Helminth Immunoglobulin G Immunology biology.protein Parasitology Antibody Follow-Up Studies |
| Zdroj: | Scopus-Elsevier ResearcherID |
| Popis: | An IgG4 ELISA based on a novel recombinant antigen was evaluated for detection of Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera from Thailand. These sera were from various groups of individuals, i.e., microfilaraemics, chronic patients, endemic normals, non-endemic normals and individuals with other parasitic and bacterial infections. Based on a cut-off optical density reading of 0.300, the IgG4 ELISA demonstrated specificity rates of 95.6-100%, sensitivity rates of 96-100%, positive predictive values of 75-100% and negative predictive values of 98.9-100%. These evaluation studies demonstrated the high specificity and sensitivity of this test for the detection of active B. malayi infection. Thus, the IgG4 ELISA would be very useful as a tool in diagnosis and in elimination programmes for brugian filariasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|