The involvement of epithelial Fas in a human model of graft versus host disease
| Autor: | Francesca Chiodi, Matthew Collin, Graham Jackson, Hans-Peter Ekre, Anne M. Dickinson, Xiao-Nong Wang, Nicolas Ruffin, Lyda M. Osorio, S Ahmed |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Fas Ligand Protein Graft vs Host Disease chemical and pharmacologic phenomena Fas ligand Pathogenesis immune system diseases Immunopathology medicine Animals Humans Transplantation Homologous fas Receptor Skin Transplantation business.industry Hematopoietic Stem Cell Transplantation Fas receptor medicine.disease Up-Regulation surgical procedures operative Graft-versus-host disease Apoptosis Immunology Immunohistochemistry Female business |
| Zdroj: | Transplantation. 91(9) |
| ISSN: | 1534-6080 |
| Popis: | Background. Graft-versus-host disease (GVHD) is an important complication occurring after hematopoietic stem-cell transplantation (HSCT). Animal model studies have shown the involvement of the Fas (APO-1/CD95)/Fas-Ligand pathway in GVHD pathogenesis, but its association with cutaneous GVHD in human remains to be established. Methods. In the present study, Fas involvement in skin damage was assessed using a human skin explant model of GVHD. Fas and FasL expression were measured by immunohistochemistry and blockade of Fas pathway was investigated using an antagonistic anti-human Fas monoclonal antibody. In addition, levels of soluble Fas (sFas) were determined in the serum of patients receiving allogeneic HSCT with and without GVHD. Results. The results showed that Fas up-regulation in the epithelium of human skin explants correlated with graft-versus-host reaction (GVHR) in the skin explant model (P |
| Databáze: | OpenAIRE |
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