Detection of TMPRSS2-ERG fusion gene in benign prostatic hyperplasia
| Autor: | S. Velaeti, A. Savvani, K. Kontogianni-Katsarou, G. Pantazi, Theoni Trangas, K. Petraki, S. Stefanakis, E. Sdrolia, N. Pandis, E. Dimitriadis |
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| Rok vydání: | 2014 |
| Předmět: |
Male
PCA3 Intraepithelial neoplasia Pathology medicine.medical_specialty Oncogene Proteins Fusion genetic structures Reverse Transcriptase Polymerase Chain Reaction Prostatic Hyperplasia General Medicine Chimeric gene Middle Aged Hyperplasia Biology urologic and male genital diseases medicine.disease Fusion gene Prostate cancer medicine.anatomical_structure Prostate Cancer research medicine Humans Gene In Situ Hybridization Fluorescence |
| Zdroj: | Tumor Biology. 35:9597-9602 |
| ISSN: | 1423-0380 1010-4283 |
| DOI: | 10.1007/s13277-014-2250-0 |
| Popis: | The Ets-related gene fusions are among the most common molecular alterations in prostate cancer (PCa) and are detected in more than 50 % of PCas. Transmembrane protease serine 2 and Ets-related gene fusion (TMPRSS2-ERG) is the most frequently identified chimeric gene and has been associated with undifferentiated and invasive phenotypes. TMPRSS2-ERG has also been detected in prostate intraepithelial neoplasia (PIN) lesions and more rarely in benign prostatic hyperplasia (BPH) regions mainly in PCa-bearing glands. The possibility that the fusion TMPRSS2-ERG may be present in BPH samples in the absence of apparent PCa was addressed. Out of 115 BPH samples, three were found positive employing RT-PCR. The presence of the fusion gene was confirmed by FISH for these samples, and an additional four samples were found to carry the TMPRSS2-ERG fusion out of 43 tested by the later approach. The presence of the TMPRSS2-ERG fusion did not result in altered expression of 12 putative downstream targets. These findings indicate that TMPRSS2-ERG may or may not lead to PCa development. |
| Databáze: | OpenAIRE |
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