Magnetic nanoparticles for imaging dendritic cells
| Autor: | Jingping Xie, Richard A. Baheza, Jared G. Cobb, John C. Gore, Wellington Pham, Saho Kobukai, Amelie Gillman, Denny Kerns, Mark D. Does, Dmitry S. Koktysh, John Virostko |
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| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Contrast Media Nanoparticle Ferric Compounds Sensitivity and Specificity Article Mice chemistry.chemical_compound In vivo medicine Animals Radiology Nuclear Medicine and imaging Particle Size Cells Cultured Staining and Labeling Chemistry Reproducibility of Results Dendritic Cells Magnetic Resonance Imaging Mice Inbred C57BL Polylysine Biophysics Nanoparticles Magnetic nanoparticles Particle size Lymph Preclinical imaging Homing (hematopoietic) |
| Zdroj: | Magnetic Resonance in Medicine. 63:1383-1390 |
| ISSN: | 0740-3194 |
| Popis: | We report the development of superparamagnetic iron oxide (SPIOs) nanoparticles and investigate the migration of SPIO-labeled dendritic cells (DCs) in a syngeneic mouse model using magnetic resonance (MR) imaging. The size of the dextran-coated SPIO is roughly 30 nm, and the DCs are capable of independent uptake of these particles, although not at levels comparable to particle uptake in the presence of a transfecting reagent. On average, with the assistance of polylysine, the particles were efficiently delivered inside DCs within one hour of incubation. The SPIO particles occupy approximately 0.35% of cell surface and are equivalent to 34.6 pg of iron per cell. In vivo imaging demonstrated that the labeled cells migrated from the injection site in the footpad to the corresponding popliteal lymph node. The homing of labeled cells in the lymph nodes resulted in a signal drop of up to 79%. Furthermore, labeling DCs with SPIO particles did not compromise cell function, we demonstrated that SPIO-enhanced MR imaging can be used to track the migration of DCs effectively in vivo. Magn Reson Med 63:1383–1390, 2010. |
| Databáze: | OpenAIRE |
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