Conformation of the calmodulin-binding domain of metabotropic glutamate receptor subtype 7 and its interaction with calmodulin
| Autor: | Yoshinori Iida, Michio Yazawa, Akiko Nakatomi, Shinya Ohki, Nobuaki Nemoto, Noriyoshi Isozumi |
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| Rok vydání: | 2011 |
| Předmět: |
Binding Sites
animal structures Calmodulin biology Calmodulin binding domain Metabotropic glutamate receptor 5 Chemistry Stereochemistry Circular Dichroism Metabotropic glutamate receptor 7 Metabotropic glutamate receptor 6 General Medicine Surface Plasmon Resonance Receptors Metabotropic Glutamate Biochemistry Protein Structure Tertiary Metabotropic glutamate receptor biology.protein Metabotropic glutamate receptor 1 Calcium Metabotropic glutamate receptor 2 Nuclear Magnetic Resonance Biomolecular Molecular Biology Protein Binding |
| Zdroj: | The Journal of Biochemistry. 149:463-474 |
| ISSN: | 1756-2651 0021-924X |
| Popis: | Calmodulin (CaM), a Ca(2+)-binding protein, is a well-known regulator of various cellular functions. One of the targets of CaM is metabotropic glutamate receptor 7 (mGluR7), which serves as a low-pass filter for glutamate in the pre-synaptic terminal to regulate neurotransmission. Surface plasmon resonance (SPR), circular dichroism (CD) spectroscopy and nuclear magnetic spectroscopy (NMR) were performed to study the structure of the peptides corresponding to the CaM-binding domain of mGluR7 and their interaction with CaM. Unlike well-known CaM-binding peptides, mGluR7 has a random coil structure even in the presence of trifluoroethanol. Moreover, NMR data suggested that the complex between Ca(2+)/CaM and the mGluR7 peptide has multiple conformations. The mGluR7 peptide has been found to interact with CaM even in the absence of Ca(2+), and the binding is directed toward the C-domain of apo-CaM rather than the N-domain. We propose a possible mechanism for the activation of mGluR7 by CaM. A pre-binding occurs between apo-CaM and mGluR7 in the resting state of cells. Then, the Ca(2+)/CaM-mGluR7 complex is formed once Ca(2+) influx occurs. The weak interaction at lower Ca(2+) concentrations is likely to bind CaM to mGluR7 for the fast complex formation in response to the elevation of Ca(2+) concentration. |
| Databáze: | OpenAIRE |
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