Identification on Mantle Cell Lymphoma Using CD20 and CD5 Coupled Upconversion Fluorescent Nanoprobes
| Autor: | Zhanbo Liu, Jialiang Wang, Qiang Lü, Yang Gao, Zaishun Jin, Jianbo Yu, Yukuan Feng, Mingkai Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science Article Subject medicine.drug_class Nanoparticle 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology medicine.disease Monoclonal antibody 01 natural sciences Fluorescence Photon upconversion 0104 chemical sciences Raji cell immune system diseases hemic and lymphatic diseases lcsh:Technology (General) medicine Biophysics lcsh:T1-995 General Materials Science Mantle cell lymphoma CD5 0210 nano-technology |
| Zdroj: | Journal of Nanomaterials, Vol 2018 (2018) |
| ISSN: | 1687-4110 |
| DOI: | 10.1155/2018/3893761 |
| Popis: | In order to determine a particular tumor cell via nanomaterials, we introduce the preparation of CD20 and CD5 coupled nanoprobes (denoted as CD20 and CD5 nanoprobes for convenience) and an application in identification of mantle cell lymphoma (MCL) from B-cell lymphoma. In this work, CD20 and CD5 nanoprobes were prepared by selectively oxidizing the carbon-carbon double bonds of oleate ligands on the surfaces of NaYF4:Yb3+,Tm3+and NaYF4:Yb3+,Er3+nanoparticles and, respectively, coupling carboxyl groups on the particles’ surfaces with CD20 and CD5 monoclonal antibodies through EDC/NHS crosslinking agents. After in situ hybridized Jeko-1 cells and Raji cells as a reference with CD20 and CD5 nanoprobes,in vitrodouble-color upconversion fluorescence imaging of Jeko-1 cells was demonstrated through visualization of blue and green fluorescence under a 980 nm laser excitation. Moreover,in vivoupconversion fluorescence imaging of the transplanted cancer model was also measured. These experimental results indicate that Jeko-1 cells have been specifically labeled by CD20 and CD5 nanoprobes. It is therefore concluded that CD20 and CD5 nanoprobes could be used to specially differentiate mantle cell lymphoma (MCL) from B-cell lymphoma. |
| Databáze: | OpenAIRE |
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