Glomerular and tubular function during AT1 receptor blockade in pigs with neonatal induced partial ureteropelvic obstruction

Autor: Lene Fogt Paulsen, Anni Eskild-Jensen, Jørgen Frøkiær, Klaus Thomsen, Yazan F. Rawashdeh, Søren Nielsen, Jens R. Nyengaard, Christine Rungø, Luis Sigaard Ferreira, Jens Christian Djurhuus
Rok vydání: 2006
Předmět:
Proton-Translocating ATPases/metabolism
Aquaporin 2/metabolism
Physiology
Kidney Glomerulus
Sus scrofa
Tetrazoles
Kidney/drug effects
urologic and male genital diseases
Kidney
Glomerular Filtration Rate/drug effects
Ion Transport/drug effects
Enzyme Inhibitors
Enzyme Inhibitors/pharmacology
Kidney Medulla/drug effects
Kidney Medulla
Benzimidazoles/pharmacology
Chemistry
Proton-Translocating ATPases
medicine.anatomical_structure
Kidney Tubules
NG-Nitroarginine Methyl Ester
Aquaporin 2
Female
Glomerular Filtration Rate
Ureteral Obstruction
medicine.medical_specialty
Kidney Cortex
Urinary system
Nitric Oxide Synthase/antagonists & inhibitors
Urology
Renal function
Kidney Glomerulus/drug effects
Internal medicine
Angiotensin II Type 1 Receptor Blockers/pharmacology
Ureteral Obstruction/metabolism
medicine
Animals
NG-Nitroarginine Methyl Ester/pharmacology
Kidney Tubules/drug effects
Hydronephrosis
Water/metabolism
Ion Transport
urogenital system
Biphenyl Compounds
Sodium
Water
medicine.disease
Tetrazoles/pharmacology
Endocrinology
Sodium/metabolism
Decreased glomerular filtration rate
Animals
Newborn
Benzimidazoles
Nitric Oxide Synthase
Angiotensin II Type 1 Receptor Blockers
Kidney disease
Kidney Cortex/drug effects
Zdroj: Eskild-Jensen, A, Thomsen, K, Rungø, C, Ferreira, L S, Paulsen, L F, Rawashdeh, Y F, Nyengaard, J R, Nielsen, S, Djurhuus, J C & Frøkiaer, J 2007, ' Glomerular and tubular function during AT1 receptor blockade in pigs with neonatal induced partial ureteropelvic obstruction ', American journal of physiology. Renal physiology, vol. 292, no. 3, pp. F921-9 . https://doi.org/10.1152/ajprenal.00407.2006
Eskild-Jensen, A, Thomsen, K, Rungø, C, Ferreira, L S, Paulsen, L F, Rawashdeh, Y F, Nyengaard, J R, Nielsen, S, Djurhuus, J C & Frøkiaer, J 2007, ' Glomerular and tubular function during AT1 receptor blockade in pigs with neonatal induced partial ureteropelvic obstruction ', American Journal of Physiology-Renal Physiology, vol. 292, no. 3, pp. F921-9 . https://doi.org/10.1152/ajprenal.00407.2006
ISSN: 1931-857X
DOI: 10.1152/ajprenal.00407.2006
Popis: Previously, we showed that neonatal induced chronic partial unilateral ureteral obstruction (PUUO) of the multipapillary pig kidney decreased glomerular filtration rate (GFR) of the obstructed kidney. We hypothesized that ANG II and nitric oxide (NO) are important for the changes in renal function and in the present study we examined the effects of chronic AT1 receptor blockade using CV-11974 (0.12 mg/h candesartan from age 23 to 30 days) on kidney function development after PUUO was induced in 2-day-old piglets. Moreover, the effect of superimposed acute NO inhibition using NG-nitro-l-arginine methyl ester (l-NAME; 15 mg/kg) was examined to identify if this has diagnostic potential. PUUO significantly increased GFR in the nonobstructed contralateral kidney independent of candesartan. In candesartan-treated piglets, the l-NAME-induced GFR reduction seen in normal and nonobstructed kidneys was absent in the partial obstructed kidneys. Urine output and fractional excretion of water were increased from the partial obstructed kidneys. Consistent with this immunohistochemical analyses showed a reduced aquaporin-2 labeling in the collecting duct principal cells. Moreover, renal sodium handling was compromised by PUUO evidenced by an increased fractional excretion of sodium which was enhanced by candesartan treatment. In conclusion, our findings suggest that the counterbalance between AT1 receptor-mediated vasoconstriction and NO-mediated vasodilatation which maintain GFR in normal young porcine kidneys is changed by neonatal induced chronic PUUO. This may have diagnostic potential in children with suspected congenital obstruction. Our results also demonstrate compromised tubular functions in response to chronic PUUO despite preservation of glomerular function.
Databáze: OpenAIRE
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