Protein–Poly(amino acid) Complex Precipitation for High-Concentration Protein Formulation
| Autor: | Tomoaki Kimoto, Kenji Handa, Takuya Maruyama, Izaki Shunsuke, Kentaro Shiraki, Takaaki Kurinomaru |
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| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Chromatography Precipitation (chemistry) Polyglutamic acid Proteins Pharmaceutical Science Polyelectrolyte Amino acid chemistry.chemical_compound Enzyme Polyglutamic Acid Solubility chemistry Biochemistry Ionic strength Native state Chemical Precipitation Polylysine |
| Zdroj: | Journal of Pharmaceutical Sciences. 103:2248-2254 |
| ISSN: | 0022-3549 |
| DOI: | 10.1002/jps.24025 |
| Popis: | A method for concentration of protein solutions is required for high-dosage protein formulation. Here, we present a precipitation–redissolution method by poly(amino acid) for proteins, including therapeutic enzymes, antibodies, and hormones. The proteins were fully precipitated by the addition of poly-l-lysine or poly-l-glutamic acid at low ionic strength, after which precipitate was dissolved at physiological ionic strength. The activities and secondary structures of redissolved proteins, especially antibodies, were almost identical to the native state. The precipitation–redissolution method is a simple and rapid technique for concentration of protein formulations. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:2248–2254, 2014 |
| Databáze: | OpenAIRE |
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