Sphingolipid Effects on the Plasma Membrane Produced by Addition of Fumonisin B1 to Maize Embryos
| Autor: | Silvia Palacios-Bahena, Liliana Noyola-Martínez, Mariana Saucedo-García, Christian Vázquez-Vázquez, Nora Gutiérrez-Nájera, Marina Gavilanes-Ruiz, Mohammed El-Hafidi, Laura Carmona-Salazar, Javier Plasencia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Ceramide animal structures Endogeny Plant Science Fusarium verticillioides 01 natural sciences plant plasma membrane Article H+-ATPase 03 medical and health sciences chemistry.chemical_compound fumonisin B1 Membrane fluidity Mycotoxin Ceramide synthase Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences Fumonisin B1 sphingolipids Ecology Endoplasmic reticulum maize embryos Botany food and beverages Sphingolipid long chain bases chemistry Biochemistry QK1-989 010606 plant biology & botany |
| Zdroj: | Plants Volume 9 Issue 2 Plants, Vol 9, Iss 2, p 150 (2020) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants9020150 |
| Popis: | Fumonisin B1 is a mycotoxin produced by Fusarium verticillioides that modifies the membrane properties from animal cells and inhibits complex sphingolipids synthesis through the inhibition of ceramide synthase. The aim of this work was to determine the effect of Fumonisin B1 on the plant plasma membrane when the mycotoxin was added to germinating maize embryos. Fumonisin B1 addition to the embryos diminished plasma membrane fluidity, increased electrolyte leakage, caused a 7-fold increase of sphinganine and a small decrease in glucosylceramide in the plasma membrane, without affecting phytosphingosine levels or fatty acid composition. A 20%&ndash 30% inhibition of the plasma membrane H+-ATPase activity was observed when embryos were germinated in the presence of the mycotoxin. Such inhibition was only associated to the decrease in glucosylceramide and the addition of exogenous ceramide to the embryos relieved the inhibition of Fumonisin B1. These results indicate that exposure of the maize embryos for 24 h to Fumonisin B1 allowed the mycotoxin to target ceramide synthase at the endoplasmic reticulum, eliciting an imbalance of endogenous sphingolipids. The latter disrupted membrane properties and inhibited the plasma membrane H+-ATPase activity. Altogether, these results illustrate the mode of action of the pathogen and a plant defense strategy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|