Supplementary Figures 1- 7 from ILT3 (LILRB4) Promotes the Immunosuppressive Function of Tumor-Educated Human Monocytic Myeloid-Derived Suppressor Cells

Autor: Philip E. Brandish, Michael Rosenzweig, Sheila H. Ranganath, Derek Y. Chiang, Michael Meehl, Chunsheng Zhang, Andrey Loboda, Barbara Joyce-Shaikh, Yujie Qu, Jun Zhang, Peter Stivers, Sarah Javaid, Jeff Grein, Anannya Bhattacharya, Eric S. Muise, Latika Singh
Rok vydání: 2023
Popis: Figure S1A-C - Dot plots and histograms from flow cytometry analysis of SK-MEL-5-educated M-MDSCs and PBMCs cultured in medium alone.Figure S1D - FACS result of cell surface staining of cytokine-induced M-MDSCs and PBMCs cultured in medium alone.Figure S1E - Cell surface staining of monocytes cultured in the presence of M-CSF and IL4 and SK-MEL-5-educated M-MDSCs by staining with HLA-DR.Figure S2 A-B - ILT3 is highly expressed on CD45+CD33+CD14+ myeloid cells in SK-MEL-5 tumor-infiltrating lymphocytes.Figure S3 - Single-cell RNAseq (scRNAseq) of human immune cells in non-small cell lung cancer (NSCLC) public dataset.Figure S4 - Association of LILRB4 expression with deconvoluted cell fractions for human tumor types in the TCGA database.Figure S5 - Suppression of autologous CD8+ T cell proliferation by cytokine-induced M-MDSCs.Figure S6 - CD33+ cells from PBMCs cultured in medium alone are not suppressive.Figure S7 - Differentially expressed genes (DEG) between SK-MEL-5 educated M-MDSCs and monocytes.
Databáze: OpenAIRE