Effect of cholera toxin on histamine release from bone marrow-derived mouse mast cells
| Autor: | Fumikazu Okajima, Ramadan I. Sha'Afi, Hirohisa Saito, Teruko Ishizaka, Thaddeus F. P. Molski, Michio Ui |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Cholera Toxin
medicine.medical_specialty Bone Marrow Cells Arachidonic Acids In Vitro Techniques Phosphatidylinositols Immunoglobulin E Pertussis toxin medicine.disease_cause Histamine Release Mice chemistry.chemical_compound Histamine H2 receptor GTP-Binding Proteins Internal medicine Cyclic AMP medicine Animals Mast Cells Adenosine Diphosphate Ribose Arachidonic Acid Multidisciplinary Phospholipase C biology Cholera toxin Thrombin Inositol trisphosphate Mast cell Molecular biology Endocrinology medicine.anatomical_structure chemistry biology.protein Secretory Rate Histamine Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 85:2504-2508 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.85.8.2504 |
| Popis: | Bone marrow-derived mouse mast cells were sensitized with monoclonal mouse IgE antibody and treated with cholera toxin (CT), which ADP-ribosylated the alpha-subunit of the stimulatory guanine nucleotide-binding regulatory protein Gs, prior to challenge with either antigen or thrombin. The CT treatment increased intracellular cAMP levels, but neither enhanced nor inhibited antigen-induced histamine release or arachidonate release. The same treatment of the sensitized bone marrow-derived mouse mast cells with CT markedly enhanced thrombin-induced histamine release without affecting arachidonate release. The CT treatment failed to affect antigen-induced and thrombin-induced generation of inositol trisphosphate and of diacylglycerol or mobilization of intracellular Ca2+. The results indicate that Gs in bone marrow-derived mouse mast cells is not involved in the transduction of the antigen-induced or thrombin-induced triggering signal to phospholipase C, which initiates the enhancement of phosphatidylinositol turnover. The enhancement of thrombin-induced histamine release by CT treatment with the observations that thrombin-induced histamine release was inhibited by pretreatment of the cells with pertussis toxin suggest that the involvement of a guanine nucleotide-binding regulatory protein in thrombin-induced biochemical events is an event distal to Ca2+ mobilization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|