Avian malaria on Madagascar: bird hosts and putative vector mosquitoes of different Plasmodium lineages
| Autor: | Ute Mackenstedt, Friederike Woog, Fano José Randrianambinintsoa, Anke Dinkel, Sébastien Boyer, Michaël Luciano Tantely, Sandrine Schmid |
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| Přispěvatelé: | Universität Hohenheim, Unité d'Entomologie Médicale [Antananarivo, Madagascar] (IPM), Institut Pasteur de Madagascar, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université de Reims Champagne-Ardenne (URCA), Staatliches Museum für Naturkunde Stuttgart (SMNS) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Leucocytozoon Malaria Avian 030231 tropical medicine Zoology Mosquito Vectors DNA Mitochondrial Polymerase Chain Reaction Plasmodium Birds 03 medical and health sciences Ploceidae 0302 clinical medicine Avian malaria parasitic diseases Madagascar medicine Animals [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology Anopheles mascarensis Uranotaenia [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health biology Research Anopheles Pycnonotidae Cytochromes b DNA Protozoan Haemosporida biology.organism_classification medicine.disease Bulbul Virology 3. Good health [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology 030104 developmental biology Infectious Diseases Haemoproteus Vector (epidemiology) Parasitology |
| Zdroj: | Parasites & Vectors Parasites and Vectors Parasites and Vectors, BioMed Central, 2017, 10 (1), pp.6. ⟨10.1186/s13071-016-1939-x⟩ |
| ISSN: | 1756-3305 |
| Popis: | International audience; BACKGROUND:Avian malaria occurs almost worldwide and is caused by Haemosporida parasites (Plasmodium, Haemoproteus and Leucocytozoon). Vectors such as mosquitoes, hippoboscid flies or biting midges are required for the transmission of these parasites. There are few studies about avian malaria parasites on Madagascar but none about suitable vectors.METHODS:To identify vectors of avian Plasmodium parasites on Madagascar, we examined head, thorax and abdomen of 418 mosquitoes from at least 18 species using a nested PCR method to amplify a 524 bp fragment of the haemosporidian mitochondrial cytochrome b gene. Sequences obtained were then compared with a large dataset of haemosporidian sequences detected in 45 different bird species (n = 686) from the same area in the Maromizaha rainforest.RESULTS:Twenty-one mosquitoes tested positive for avian malaria parasites. Haemoproteus DNA was found in nine mosquitoes (2.15%) while Plasmodium DNA was found in 12 mosquitoes (2.87%). Seven distinct lineages were identified among the Plasmodium DNA samples. Some lineages were also found in the examined bird samples: Plasmodium sp. WA46 (EU810628.1) in the Madagascar bulbul, Plasmodium sp. mosquito 132 (AB308050.1) in 15 bird species belonging to eight families, Plasmodium sp. PV12 (GQ150194.1) in eleven bird species belonging to eight families and Plasmodium sp. P31 (DQ839060.1) was found in three weaver bird species.CONCLUSION:This study provides the first insight into avian malaria transmission in the Maromizaha rainforest in eastern Madagascar. Five Haemoproteus lineages and seven Plasmodium lineages were detected in the examined mosquitoes. Complete life-cycles for the specialist lineages WA46 and P31 and for the generalist lineages mosquito132 and PV12 of Plasmodium are proposed. In addition, we have identified for the first time Anopheles mascarensis and Uranotaenia spp. as vectors for avian malaria and offer the first description of vector mosquitoes for avian malaria in Madagascar. |
| Databáze: | OpenAIRE |
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