Cryopreservation of very low numbers of spermatozoa from male patients undergoing infertility treatment using agarose capsules
| Autor: | Hiroyuki Abe, Shota Hatakeyama, Susumu Tokuoka, Yasuhisa Araki, Yasuyuki Araki |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Infertility endocrine system Cancer Research Cryoprotectant medicine.medical_treatment Motility Capsules Semen Biology Cryopreservation Intracytoplasmic sperm injection Andrology 03 medical and health sciences chemistry.chemical_compound Cryoprotective Agents 0302 clinical medicine medicine Humans Sperm Injections Intracytoplasmic Infertility Male reproductive and urinary physiology 030219 obstetrics & reproductive medicine urogenital system Sepharose Cell Biology Anatomy medicine.disease Spermatozoa Solutions 030104 developmental biology chemistry Male patient Sperm Motility Agarose |
| Zdroj: | Human Cell. 30:201-208 |
| ISSN: | 1749-0774 |
| DOI: | 10.1007/s13577-017-0166-x |
| Popis: | This study tried to cryopreserve low numbers of spermatozoa from men undergoing infertility treatments by inserting into agarose capsules. The capsules were transferred into a drop of cryoprotectant solution and injected 3–4 motile spermatozoa that were selected by the swim-up method by conventional intracytoplasmic sperm injection. These capsules were put on a Cryotop® and frozen in liquid nitrogen vapor, and then submerged into liquid nitrogen and subsequently thawed and recovered. The motile spermatozoa in the capsules were counted. Eventually, we cryopreserved 2142 motile spermatozoa in 702 agarose capsules from 26 male patients and 1356 (63%) spermatozoa maintained their motility after thawing. The spermatozoa motility rates after thawing (MRAT) ranged from 20.0% (5/25) to 95.1% (58/61) among patients. The median MRAT was 68.3% (interquartile range 46.1–75.7). The total number of motile spermatozoa collected by swim-up method strongly correlated with MRAT (r = 0.746). It was possible to cryopreserve spermatozoa from male patients undergoing infertility treatment using agarose capsules. However, there were wide differences in MRAT among patients. It seems the spermatozoa from semen where there were many motile spermatozoa may have higher freezing resistance. Further studies using this method in cryptozoospermic semen, testicular and epididymal spermatozoa are required. |
| Databáze: | OpenAIRE |
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