Cellulose-based hydrogel on quantum dots with molecularly imprinted polymers for the detection of CA19-9 protein cancer biomarker

Autor: Ana Margarida L. Piloto, David S. M. Ribeiro, S. Sofia M. Rodrigues, João L. M. Santos, Paula Sampaio, Maria Goreti Ferreira Sales
Přispěvatelé: Universidade do Minho
Rok vydání: 2022
Předmět:
Zdroj: Microchimica Acta. 189
ISSN: 1436-5073
0026-3672
DOI: 10.1007/s00604-022-05230-8
Popis: The online version contains supplementary material available at: https://doi.org/10.1007/s00604-022-05230
Molecularly imprinted polymers MIPs were successfully assembled around quantum dots (QDs), for the detection of the protein biomarker CA19-9 associated to pancreatic cancer (PC). These imprinted materials MIP@QDs were incorporated within the cellulose hydrogel with retention of its conformational structure inside the binding cavities. The concept is to use MIPs which function as the biorecognition elements, conjugated to cadmium telluride QDs as the sensing system. The excitation wavelength was set to 477 nm and the fuorescence signal was measured at its maximum intensity, with an emission range between 530 and 780 nm. The fuorescence quenching of the imprinted cellulose hydrogels occurred with increasing concentrations of CA19-9, showing linearity in the range 2.76×10 −2 – 5.23×10 2 U/ml, in a 1000-fold diluted human serum. Replicates of the imprinted hydrogel show a linear response below the cut-of values for pancreatic cancer diagnosis (
The authors acknowledge the fnancial support through project 3C´s (PTDC/AAG TEC/5400/2014). Ana Margarida L. Piloto also acknowledges Fundação para a Ciência e Tecnologia, I.P., for the grant reference SFRH/BPD/116067/2016, co-funded by Programa Operacional Regional Norte 2020, from the European Union. David S. M. Ribeiro thanks FCT (Fundação para a Ciência e Tecnologia) for funding through program DL 57/2016 Norma transitória. The authors acknowledge also the support of i3S Scientifc Platforms Advanced Light Microscopy and HEMS, members of the national infrastructure PPBI—Portuguese Platform of Bioimaging (PPBI-POCI-01-0145-FEDER-022122).
info:eu-repo/semantics/publishedVersion
Databáze: OpenAIRE
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