Immunomodulatory and anticancer potential of Gan cao (Glycyrrhiza uralensis Fisch.) polysaccharides by CT-26 colon carcinoma cell growth inhibition and cytokine IL-7 upregulation in vitro
| Autor: | Peter Amwoga Ayeka, Yuhong Bian, Peter Mwitari, Yanjun Zhang, Xiaoqian Chu, Rosette Uzayisenga, Elick O. Otachi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Immunomodulatory medicine.medical_treatment Antineoplastic Agents Polysaccharide Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Polysaccharides Cell Line Tumor medicine Animals Glycyrrhiza uralensis Cytotoxicity Cell Proliferation chemistry.chemical_classification IL-7 Traditional medicine biology Plant Extracts Anti-cancer Cell growth Interleukin-7 General Medicine biology.organism_classification Molecular biology In vitro Up-Regulation TCM 030104 developmental biology Cytokine Complementary and alternative medicine chemistry 030220 oncology & carcinogenesis Colonic Neoplasms Cancer cell Growth inhibition Research Article |
| Zdroj: | BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| DOI: | 10.1186/s12906-016-1171-4 |
| Popis: | Background Chinese licorice, (Glycyrrhiza uralensis Fisch.) is one of the commonly prescribed herbs in Traditional Chinese Medicine (TCM). Gancao, as commonly known in China, is associated with immune-modulating and anti-tumor potential though the mechanism of action is not well known. In this study, we investigated the in vitro immunomodulatory and antitumor potential of Glycyrrhiza uralensis polysaccharides fractions of high molecular weight (fraction A), low molecular weight (fraction B) and crude extract (fraction C). Methods Cell proliferation and cytotoxicity was investigated using Cell Counting kit 8 (CCK-8) on Intestinal epithelial cell line (IEC-6) and Colon carcinoma cell line (CT-26). IL-7 gene expression relative to GAPDH was analysed using Real time PCR. The stimulation and viability of T lymphocytes was determined by Trypan blue exclusion assay. Results G.uralensis polysaccharides did not inhibit proliferation of IEC-6 cells even at high concentration. The ED50 was found to be 100 μg/ml. On the other hand, the polysaccharides inhibited the proliferation of cancer cells (CT-26) at a concentration of ≤50 μg/ml. Within 72 h of treatment with the polysaccharides, expression of IL-7 gene was up-regulated over 2 times. It was also noted that, IEC-6 cells secrete IL-7 cytokine into media when treated with G.uralensis polysaccharides. The secreted IL-7 stimulated proliferation of freshly isolated T lymphocytes within 6 h. The effect of the polysaccharides were found to be molecular weight depended, with low molecular weight having a profound effect compared to high molecular weight and total crude extract. Conclusion Our findings indicate that G.uralensis polysaccharides especially those of low molecular weight have a potential as anticancer agents. Of great importance, is the ability of the polysaccharides to up-regulate anticancer cytokine IL-7, which is important in proliferation and maturation of immune cells and it is associated with better prognosis in cancer. Therefore, immunomodulation is a possible mode of action of the polysaccharides in cancer therapy. Electronic supplementary material The online version of this article (doi:10.1186/s12906-016-1171-4) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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