Determination of sunscreen immune protection factors using human dendritic cell suspensions
| Autor: | Catherine Dalbiez-Gauthier, D. Schmitt, B. Le Varlet, Pascal Courtellemont, J. Peguet-Navarro |
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| Rok vydání: | 2004 |
| Předmět: |
Ultraviolet Rays
T-Lymphocytes medicine.medical_treatment Drug Evaluation Preclinical Human skin Cell Separation Pharmacology Biology Lymphocyte Activation Toxicology medicine Humans Cells Cultured Skin Immunosuppression Therapy integumentary system Dose-Response Relationship Radiation Immunosuppression Dendritic Cells General Medicine Dendritic cell Mixed lymphocyte reaction In vitro Dose–response relationship Cytoprotection Langerhans Cells Photoprotection Immunology Lymphocyte Culture Test Mixed Sunscreening Agents Explant culture |
| Zdroj: | Toxicology in Vitro. 18:359-364 |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2003.10.005 |
| Popis: | In a previous study, we have used UVB-irradiated human skin explants and the allostimulatory function of Langerhans cells (LC) to determine immune protection factors (IPF) for sunscreens. We sought here to simplify the model by using either human enriched LC suspensions or in vitro generated dendritic cells from human monocytes (MoDC). LC or MoDC suspensions were irradiated with increasing doses of UVB through a piece of translucent strip recovered or not with the sunscreens. The allostimulatory function of the cells was then analysed in a mixed lymphocyte reaction and the UVB dose providing 50% immunosuppression (D50%) was determined graphically. IPF were determined by the ratio of the D50% value in the presence of sunscreen to that of the vehicle alone. In either experimental conditions, the D50% in the presence of sunscreens was significantly higher (p < 0.01) than that obtained with the vehicle, demonstrating the sunscreen immunoprotective effect. IPF values obtained with either DC suspensions were very similar and quite comparable to those previously obtained in the skin explant model. Thus, the present in vitro model provides easy tools to determine a new important biological parameter for sunscreens, i.e. immune protection. |
| Databáze: | OpenAIRE |
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