Rep-PCR characterization and biochemical selection of lactic acid bacteria isolated from the Delta area of Egypt
| Autor: | Mariam Mohammed, Nadia Omran, Shimaa Anwar, Sameh Awad, Morsi El-Soda, Hossam Abd El-Aziz |
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| Rok vydání: | 2009 |
| Předmět: |
Lactobacillus paracasei
Lactobacillus fermentum Lactococcus Aminopeptidases Polymerase Chain Reaction Microbiology Species Specificity Lactobacillus Animals Cluster Analysis Humans biology Polysaccharides Bacterial Lactococcus lactis Genetic Variation food and beverages General Medicine Hydrogen-Ion Concentration biology.organism_classification DNA Fingerprinting Milk Enterococcus Food Microbiology bacteria Egypt Dairy Products Lactobacillus plantarum Food Science Enterococcus faecium |
| Zdroj: | International Journal of Food Microbiology. 128:417-423 |
| ISSN: | 0168-1605 |
| DOI: | 10.1016/j.ijfoodmicro.2008.09.022 |
| Popis: | Samples of raw milk and traditional dairy products were collected from different rural areas in the Delta region. 170 isolates from these products were identified using repetitive genomic element-PCR (Rep-PCR) fingerprinting. The identified isolates were tested for efficiency of biomass production and separation, acidifying activity, autolytic and aminopeptidase properties, antagonistic activities and exopolysaccharide production. The obtained results revealed that the Lactobacillus delbrueckii subsp. lactis, Lactobacillus fermentum, Enterococcus faecium Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus paracasei subsp. paracasei, Lactobacillus plantarum and Lactococcus lactis subsp. lactis were the predominant species in Egyptian dairy products. Two percent of Lactococcus, 10% of Lactobacillus and 1% of Enterococcus isolates showed fast acidifying activity. Aminopeptidase and autolytic properties were generally higher for most Lactobacillus strains when compared to other strains. Among these species, lactobacillus paracasei subsp. paracasei was the highest in Aminopeptidase activity and autolytic properties. Antagonistic activity was detected in 40% of Lactococcus, 70% of Lactobacillus and 50% of Enterococcus isolates. Some isolates produced exopolysaccharides in milk and dairy products. |
| Databáze: | OpenAIRE |
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