Patterns of Wnt/Fzd/LRP gene expression during embryonic hematopoiesis
| Autor: | Edwina Dobbin, Pamela M. Corrigan, Robin Freeburn, Helen Wheadon |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Swine
Embryonic Development Stem cell factor CHO Cells Biology Mice Cricetulus Cricetinae Animals Cell Lineage Progenitor cell Embryonic Stem Cells LDL-Receptor Related Proteins Cell Proliferation Regulation of gene expression Gene Expression Profiling Wnt signaling pathway Gene Expression Regulation Developmental LRP5 Cell Differentiation Cell Biology Hematology Embryo Mammalian Embryonic stem cell Frizzled Receptors Cell biology Hematopoiesis Wnt Proteins Haematopoiesis Immunology Stem cell Developmental Biology |
| Zdroj: | Stem cells and development. 18(5) |
| ISSN: | 1557-8534 |
| Popis: | Wnt signaling plays several roles in hematopoiesis, promoting hemopoietic stem cell (HSC) self-renewal, providing proliferative signals for immature progenitors and regulating lineage commitment. To ascertain which Wnt proteins and receptors are important during hematopoietic development, we used two systems; in vitro hematopoietic differentiation of embryonic stem (ES) cells and tissues isolated from sites specific for hematopoiesis during mouse embryogenesis. Initially genes involved in hematopoiesis were profiled and indicate differentiating ES cells undergo a wave of primitive hematopoiesis (Day 3.75) similar to the mouse yolk sac, followed by a wave of more definitive hematopoiesis (Day 7.75) comparable to the aorta-gonad-mesonephros (AGM) and E15.5 liver with lineage commitment by Day 15. A similar biphasic expression pattern occurred for Wnt/Fzd/LRP genes with Wnt 3, 5a, 8a, Fzd4, and LRP5 becoming upregulated during primitive hematopoiesis, followed by Wnt3a, 6, 7b, 10b, and 16 during more definitive hematopoiesis. High expression of Wnt5a, Fzd4, and LRP5 during the first phase of hematopoiesis suggests these genes are involved in early hematopoietic regulation. Wnt3a and 16 were also expressed at specific stages, with Wnt16 detected when the earliest lymphoid progenitors are formed (AGM and 2 degrees BC of ES differentiation). Wnt3a expression corresponded with the induction of definitive hematopoiesis a period, which involves rapid expansion of HSC (Day 7.75 of ES differentiation, AGM and E15.5 liver). Supplementation with Wnt3a during ES hematopoietic differentiation increased proliferation and appeared to promote stem cell expansion. Overall this study provides valuable information on the Wnt/Fzd/LRP involved in supporting embryonic hematopoiesis. |
| Databáze: | OpenAIRE |
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