In Vitro Biomechanical Strain Regulation of Fibroblast Wound Healing
| Autor: | Paul R. Standley, Michael R. Hicks, Thanh V. Cao |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Complementary and Manual Therapy
Vacuum Pharmacology Nitric Oxide Nitric oxide chemistry.chemical_compound Downregulation and upregulation Humans Medicine Fibroblast Protein Kinase C PI3K/AKT/mTOR pathway Protein kinase C Wound Healing business.industry Kinase Fibroblasts Musculoskeletal Manipulations Biomechanical Phenomena medicine.anatomical_structure Complementary and alternative medicine chemistry Immunology Phosphatidylinositol 3-Kinase business Wound healing cGMP-dependent protein kinase |
| Zdroj: | Journal of Osteopathic Medicine. 113:806-818 |
| ISSN: | 2702-3648 |
| DOI: | 10.7556/jaoa.2013.056 |
| Popis: | Context: Strain-directed therapy such as vacuum compression and manual manipulative therapies are clinically effective, but their cellular and molecular mechanisms are not well understood. Objective: To determine the effects of modeled myofascial release (MFR) on fibroblast wound healing and to investigate the potential role of nitric oxide (NO) in mediating these responses. Methods: Using an in vitro scratch wound strain model, the authors investigated human fibroblast wound healing characteristics in response to injurious repetitive motion strain (RMS) and MFR. Secretion of NO was induced with interleukin-1β and sodium nitroprusside and inhibited with NO synthase inhibitor L-NG-monomethyl arginine citrate (L-NMMA) to determine the effects of NO on wound healing. Protein microarray was also performed to evaluate the expression of intracellular protein and activation of protein kinase G (PKG), extracellular signal-regulated kinase (ERK1/2), protein kinase C (PKC), and phosphoinositide 3-kinase (PI3K), the downstream effectors in the NO pathway. Results: Fibroblasts that received RMS resulted in reduced wound closure rates (vs nonstrain, P Conclusion: If clinically translatable, these data suggest that mechanical strain such as vacuum compression therapy and manual manipulative therapy may modify PKC and PI3K to sensitize fibroblasts to NO and improve wound healing by promoting cell proliferation and migration by means of PKC and PKG signaling. |
| Databáze: | OpenAIRE |
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