Comparison of different methods for spongin-like collagen extraction from marine sponges (Chondrilla Caribensis and Aplysina Fulva): physicochemical properties and in vitro biological aAnalysis
| Autor: | Gabriela Martins de Araújo, Antonio Miranda, Ana Claudia Muniz Renno, Amanda de Souza, Renata Neves Granito, Fábio Ruiz Simões, Flávio Lopes Alves, Na Yu, Tiago Akira Tashiro de Araújo, Márcio R. Custódio, Alan de França Santana, Anna Rafaela Cavalcante Braga |
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| Rok vydání: | 2021 |
| Předmět: |
spongin-like collagen
Marine sponges Antioxidant extraction protocols medicine.medical_treatment Aplysina fulva Filtration and Separation TP1-1185 02 engineering and technology 03 medical and health sciences Chemical engineering medicine Chemical Engineering (miscellaneous) marine sponges Chondrilla caribensis Fibroblast 030304 developmental biology 0303 health sciences Chromatography Spongin Chemistry Chemical technology Process Chemistry and Technology Extraction (chemistry) 021001 nanoscience & nanotechnology In vitro medicine.anatomical_structure TP155-156 ZOOLOGIA (CLASSIFICAÇÃO) 0210 nano-technology biomaterials |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Membranes, Vol 11, Iss 522, p 522 (2021) Membranes Volume 11 Issue 7 |
| Popis: | This study aimed to compare different protocols (Protocol 1: P1 Protocol 2: P2 Protocol 3: P3 Protocol 4: P4) for the extraction of spongin-like collagen (SC) from marine sponges. The SEM micrographs demonstrated a fibrillar structure for the extracts from Chondrilla caribensis and the nodular/particulate aggregates for Aplysina fulva. FTIR showed for all samples peaks similar to collagen for both species. For C. caribensis, the extracts obtained using P2, P3, and P4 protocols presented higher values of extraction yield, TPQ, and GAGs. P2 and P4 showed higher values of SC concentration and for antioxidant analysis. For A. fulva, P2, P3, and P4 provided a higher extraction yield besides an increase in the antioxidant assay. For both species, no difference was observed for Col quantification and TPQ analysis also, higher values of GAGs were found using P2 and P4. Fibroblast proliferation observed for C. caribensis was lower for P1 on day 1 and for P2 and P3 on day 3 (for 50%) compared to the control group. There was a significant reduction in fibroblast cell proliferation for all A. fulva extracts evaluated. It can be concluded that protocols P2 and P4 were more efficient for extracting SC from C. caribensis. |
| Databáze: | OpenAIRE |
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