NET WATER TRANSPORT VIA RAT COLON EPITELIUM UNDER THE EXPERIMENTAL DYSBIOSIS
| Autor: | N. Roslova, I. Vareniuk, O Tiapko, A Putnikov, Tetyana Beregova, L. Zakordonets, O.V. Lynchak, T Sergiychuk, T. Dovbynchuk, M. Dzerzhynsky, G Tolstanova |
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| Předmět: |
Male
medicine.medical_specialty Physiology medicine.drug_class Colon Antibiotics Administration Oral Pharmacology Azithromycin Aquaporins Gastroenterology Injections Intramuscular Intestinal absorption Cell Degranulation Intestinal mucosa In vivo Internal medicine medicine Animals Mast Cells Intestinal Mucosa Rats Wistar Cephalosporin Antibiotic Water transport Chemistry Microbiota Ceftriaxone Water Biological Transport Anti-Bacterial Agents Rats Perfusion Gene Expression Regulation Intestinal Absorption Dysbiosis medicine.drug |
| Zdroj: | Scopus-Elsevier Europe PubMed Central |
| Popis: | The aim of the present study was to investigate the effect of cephalosporin antibiotic ceftriaxone (50 mg/kg, i/m) and mac- rolide antibiotic azithromycin (15 mg/kg, per.os.) on net water transport across rat colonic epithelium. Study was done on male Wistar rats (180-250 g). Azithromycin or ceftriaxone was injected daily for 5 days. Net water transport was evaluated on the 6th day by isolated colonic loop perfusion technique in vivo on anaesthetized rats. Treatment with azithromycin increased 2,4-fold the absorption of water, while ceftriaxone caused decrease 1,9-fold water absorption. The antibiotics treatment within five days didn't change the composition of the fecal and colonic parietal microbiota. Azithromycin-induced increase in water absorption was associated with upregulation of AQP 8 water channel expression (P < 0.05) in colonic mucosa. Ceftriaxone treatment didn't change protein level of AQP8 but induced pro-inflammatory changes in colonic mucosa structure and mast cells degranulation. We showed for the first time the opposite effects ofmacrolide and cephalosporin antibiotics on net water transport across rat colonic epithelium. |
| Databáze: | OpenAIRE |
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