Polarization of Reactive Astrocytes in Response to Spinal Cord Injury is Enhanced by M2 Macrophage-Mediated Activation of Wnt/β-Catenin Pathway
Autor: | Francois Renault-Mihara, Hideyuki Okano, Masaya Nakamura, Iki Sonn |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Neuroscience (miscellaneous) Glial scar 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine In vivo Wnt3A Protein medicine Animals Spinal cord injury Wnt Signaling Pathway Spinal Cord Injuries Chemistry Chemotaxis Integrin beta1 Wnt signaling pathway Cell Polarity Macrophage Activation medicine.disease M2 Macrophage Spinal cord Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Neurology Catenin Astrocytes 030217 neurology & neurosurgery Chemotaxis assay |
Zdroj: | Molecular neurobiology. 57(4) |
ISSN: | 1559-1182 |
Popis: | Understanding the mechanisms of glial scar formation by reactive astrocytes is crucial for elaborating a therapeutic strategy to brain and spinal cord injury. However, the extrinsic mechanisms that drive the polarization of reactive astrocytes, the first step in glial scar formation, remain poorly understood. Here, using an in vitro chemotaxis assay as an experimental model for polarization, we observed that Il4-M2 macrophages are stronger inducers of reactive astrocytes' polarization, compared to naive or M1 macrophages. Then, we showed that both β1-integrin and Wnt/β-catenin pathways in astrocytes are required for this polarization in vitro and in vivo after spinal cord crush injury in mice. These findings provide molecular targets for manipulating the polarization of reactive astrocytes in order to potentially enhance the healing of SCI lesions. |
Databáze: | OpenAIRE |
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