Genetic variation of Trigonobalanus verticillata, a primitive species of Fagaceae, in Malaysia revealed by chloroplast sequences and AFLP markers
| Autor: | Abdul Latiff Mohamed, Mahani Mansor Clyde, Ko Harada, Koichi Kamiya |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Genetic Markers
education.field_of_study Chloroplasts Population Malaysia Sequence Analysis DNA General Medicine Biology Fagaceae Polymerase Chain Reaction Fixation index Nucleotide diversity Chloroplast DNA Genetic marker Mutation Genetic variation Botany Genetics Amplified fragment length polymorphism education Molecular clock Molecular Biology Phylogeny |
| Zdroj: | Genes & Genetic Systems. 77:177-186 |
| ISSN: | 1880-5779 1341-7568 |
| DOI: | 10.1266/ggs.77.177 |
| Popis: | The genetic variation of Trigonobalanus verticillata, the most recently described genus of Fagaceae, was studied using chloroplast DNA sequences and AFLP fingerprinting. This species has a restricted distribution that is known to include seven localities in tropical lower montane forests in Malaysia and Indonesia. A total of 75 individuals were collected from Bario, Kinabalu, and Fraser's Hill in Malaysia. The sequences of rbcL, matK, and three non-coding regions (atpB-rbcL spacer, trnL intron, and trnL-trnF spacer) were determined for 19 individuals from these populations. We found a total of 30 nucleotide substitutions and four length variations, which allowed identification of three haplotypes characterizing each population. No substitutions were detected within populations, while the tandem repeats in the trnL -trnF spacer had a variable repeat number of a 20-bp motif only in Kinabalu. The differentiation of the populations inferred from the cpDNA molecular clock calibrated with paleontological data was estimated to be 8.3 MYA between Bario and Kinabalu, and 16.7 MYA between Fraser's Hill and the other populations. In AFLP analysis, four selective primer pairs yielded a total of 431 loci, of which 340 (78.9%) were polymorphic. The results showed relatively high gene diversity (H(S) = 0.153 and H(T) = 0.198) and nucleotide diversity (pi(S) = 0.0132 and pi(T) = 0.0168) both within and among the populations. Although the cpDNA data suggest that little or no gene flow occurred between the populations via seeds, the fixation index estimated from AFLP data (F(ST) = 0.153 and N(ST) = 0.214) implies that some gene flow occurs between populations, possibly through pollen transfer. |
| Databáze: | OpenAIRE |
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