Limited Effector Memory B-Cell Response to Envelope Glycoprotein B During Primary Human Cytomegalovirus Infection
| Autor: | Michael Mach, Arnaud Marchant, Nicolas Dauby, Edwige Haelterman, Catherine Donner, Marie-Luce Delforge, Caroline Kummert, Sandra Lecomte, Delphine Sartori |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Human cytomegalovirus viruses Congenital cytomegalovirus infection B-Lymphocyte Subsets Cytomegalovirus Antibodies Viral Immunoglobulin G 03 medical and health sciences Antigen Viral Envelope Proteins Pregnancy medicine Immunology and Allergy Humans Memory B cell biology Toxoid virus diseases biochemical phenomena metabolism and nutrition medicine.disease Virology Antibodies Neutralizing 030104 developmental biology Infectious Diseases Phenotype Immunization Immunology Cytomegalovirus Infections biology.protein Female Antibody |
| Zdroj: | The Journal of infectious diseases. 213(10) |
| ISSN: | 1537-6613 |
| Popis: | BACKGROUND Following primary human cytomegalovirus (HCMV) infection, the production of antibodies against envelope glycoprotein B (gB) is delayed, compared with production of antibodies against tegument proteins, and this likely reduces the control of HCMV dissemination. METHODS The frequency and the phenotype of gB-specific and tegument protein-specific B cells were studied in a cohort of pregnant women with primary HCMV infection. Healthy adults who had chronic HCMV infection or were recently immunized with tetanus toxoid (TT) were included as controls. RESULTS Primary HCMV infection was associated with high and similar frequencies of gB-specific and tegument protein-specific B cells following primary HCMV infection. During primary infection, tegument protein-specific B cells expressed an activated (CD21(low)) memory B-cell (MBC) phenotype. Activated MBCs were also induced by TT booster immunization, indicating that the expansion of this subset is part of the physiological B-cell response to protein antigens. In contrast, gB-specific B cells had a predominant classical (CD21(+)) MBC phenotype during both primary and chronic infections. CONCLUSIONS The delayed production of gB-specific immunoglobulin G (IgG) during primary HCMV infection is associated with a limited induction of MBCs with effector potential. This novel mechanism by which HCMV may interfere with the production of neutralizing antibodies could represent a target for therapeutic immunization. |
| Databáze: | OpenAIRE |
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