Investigation of the role of tumour necrosis factor-α, interleukin-1β, interleukin-10, nitric oxide and rheumatoid factor-immunoglobulin M in a rat model of arthritis
| Autor: | Raida K. Al-Rukibat, Wael M. Hananeh, A Boumezrag, O Okour, Mohammad S. Khalifeh |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Necrosis medicine.medical_treatment Interleukin-1beta Arthritis Nitric Oxide Nitric oxide Arthritis Rheumatoid Rats Sprague-Dawley chemistry.chemical_compound Rheumatoid Factor medicine Animals Rheumatoid factor General Veterinary Tumor Necrosis Factor-alpha business.industry Autoantibody Th1 Cells medicine.disease Interleukin-10 Rats Disease Models Animal Interleukin 10 Cytokine Immunoglobulin M chemistry Rheumatoid arthritis Immunology Animal Science and Zoology medicine.symptom business |
| Zdroj: | Laboratory Animals. 44:143-149 |
| ISSN: | 1758-1117 0023-6772 |
| DOI: | 10.1258/la.2009.0080132 |
| Popis: | Collagen-induced arthritis (CIA) in rats is a widely used preclinical animal model of rheumatoid arthritis (RA). However, CIA development in Sprague-Dawley (SD) rats is less severe in terms of inflammatory response compared with other strains. Therefore, a modified CIA model called MCIA, using N-acetylmuramyl dipeptide (MDP), has been developed in the less sensitive SD rat strains. This work was conducted to better understand the immunopathological role and contributions of the pro-inflammatory T-helper type 1 (Th-1) cytokines and inflammatory mediators, interleukin-1 (IL-1 β), tumour necrosis factor-alpha (TNF- α) and nitric oxide (NO); the anti-inflammatory T-helper type 2 (Th-2) cytokine, IL-10 and autoantibodies such as rheumatoid factor (RF)-immunoglobulin M (IgM) in this newly developed RA model. TNF-α, NO and RF-IgM levels were significantly increased, while IL-1 β levels were not affected in this MCIA rat model. The levels of IL-10 were lower than the baseline when compared with controls. In conclusion: (1) the immunological features represented in the MCIA rat model favour the Th-1 cytokine profile over Th-2 and (2) RF-IgM can be used as a diagnostic test in preclinical RA models. |
| Databáze: | OpenAIRE |
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