Nanostraw membrane stamping for direct delivery of molecules into adhesive cells
| Autor: | Koji Nakazawa, Daisuke Miyamoto, Takeo Miyake, Yiming Shi, Bowen Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell Survival Cellular functions Cell Culture Techniques lcsh:Medicine Article Permeability 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Drug Delivery Systems Molecule Animals Nanotechnology lcsh:Science Multidisciplinary Nanoscale materials lcsh:R Membranes Artificial Stamping Fluoresceins Nanostructures Calcein Electrophysiology 030104 developmental biology Membrane chemistry Cell culture Cytoplasm Biophysics NIH 3T3 Cells Permeation and transport lcsh:Q Adhesive 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-43340-1 |
| Popis: | Delivering ions and molecules into living cells has become an important challenge in medical and biological fields. Conventional molecular delivery, however, has several issues such as physical and chemical damage to biological cells. Here, we present a method of directly delivering molecules into adhesive cells with an Au-based nanostraw membrane stamp that can physically inject a target molecule into the cytoplasm through a nanostraw duct. We successfully delivered calcein target molecules into adhesive cells with high efficiency (85%) and viability (90%). Furthermore, we modeled the molecular flow through Au nanostraws and then demonstrated the control of calcein flow by changing the concentration and geometry of Au nanostraws. Our Au membrane stamping provides a new way of accessing the cytoplasm to modulate cellular functions via injected molecules. |
| Databáze: | OpenAIRE |
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