Assessment of cytotoxic and apoptotic effects of benzaldehyde using different assays
| Autor: | Lokman Alpsoy, Z. Ulker, Ahmet Mihmanli |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cell Survival
Health Toxicology and Mutagenesis Tetrazolium Salts Apoptosis Biology Toxicology chemistry.chemical_compound Lactate dehydrogenase In Situ Nick-End Labeling Humans Cytotoxic T cell Lymphocytes Cytotoxicity Cells Cultured Cell Proliferation TUNEL assay L-Lactate Dehydrogenase Cytotoxins Cell growth General Medicine Molecular biology Terminal deoxynucleotidyl transferase chemistry Cell culture Benzaldehydes Biological Assay DNA Damage |
| Zdroj: | Human & Experimental Toxicology. 32:858-864 |
| ISSN: | 1477-0903 0960-3271 |
| DOI: | 10.1177/0960327112470271 |
| Popis: | Benzaldehyde (BA) occurs naturally in a number of plants, including cherry, fig and peach fruit and carnation flowers at therapeutic doses. In addition, it is used in cosmetics, personal care products and food as a preservative. In this study, we aimed to determine the cytotoxic and apoptotic effects of different concentrations of BA on cultured human lymphocytes using lactate dehydrogenase assay, cell proliferation (water-soluble tetrazolium salts-1) assay and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) test (apoptotic test) as a group of cytotoxicity tests at 6th and 24th h on human lymphocyte cell culture. The cytotoxicity increased when cells were treated with 10, 25 and 50 μg/mL concentrations of BA ( p |
| Databáze: | OpenAIRE |
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