Proteome and microbiota analysis highlight Lactobacillus plantarum TWK10 supplementation improves energy metabolism and exercise performance in mice
| Autor: | Wen Chyuan Chen, Chen Chung Liao, Chi Chang Huang, Ming Yi Chen, Yen Chun Huang, Yen Shuo Chiu, Yi-Ming Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
proteome butyrate‐produce bacteria Biology Gut flora Proteomics law.invention 03 medical and health sciences Probiotic 0302 clinical medicine Lipid oxidation lipid oxidation law TX341-641 Lactobacillus plantarum TWK10 Beta oxidation Nutrition. Foods and food supply Peroxisome biology.organism_classification 030104 developmental biology Biochemistry Proteome 030211 gastroenterology & hepatology Lactobacillus plantarum Food Science |
| Zdroj: | Food Science & Nutrition, 8(7):3525-3534 Food Science & Nutrition, Vol 8, Iss 7, Pp 3525-3534 (2020) |
| Popis: | Lactobacillus plantarum TWK10 (LP10) is a probiotic known to improve endurance exercise performance. Here, we analyze the proteomics and metagenomic changes in a LP10 supplemented mouse model. Male ICR mice were divided into two groups (n = 8) to receive by oral gavage either vehicle or of LP10 for 6 weeks. Proteins changes by LP10 treatment were subjected to the Ingenuity Pathway Analysis (IPA) to provide corroborative evidence for differential regulation of molecular and cellular functions affecting metabolic processes. Fecal samples were obtained from each mouse, and the microbial community profile analyzed by pyrosequencing of the 16S rRNA genes. Of the 880 identified proteins, 25 proteins were significantly downregulated and 44 proteins were significantly upregulated in the LP10 treated compared to vehicle group. LP10 supplementation shift in the gut microbiota to butyrate‐producing members and provided from lipid oxidation since peroxisomal fatty acid oxidation in liver. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|