Electron microscopic localization of lipoteichoic acid on group a streptococci
| Autor: | Barbara Wagner, Manfred Wagner, Miloš Rýc, Ruth Bicová |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Lipopolysaccharides
Streptococcus pyogenes Immunoelectron microscopy Immunology Pronase medicine.disease_cause Bacterial Adhesion Microbiology Cell Wall Concanavalin A medicine biology Streptococcus Protoplasts respiratory system Trypsin Streptococcaceae biology.organism_classification Immunohistochemistry Teichoic Acids carbohydrates (lipids) Microscopy Electron stomatognathic diseases Ferritins biology.protein Cytochemistry lipids (amino acids peptides and proteins) Gold Lipoteichoic acid medicine.drug |
| Zdroj: | Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology. 269:168-178 |
| ISSN: | 0176-6724 |
| DOI: | 10.1016/s0176-6724(88)80093-2 |
| Popis: | The location of lipoteichoic acid (LTA) on the surface of group A streptococci was studied by immunoelectron microscopic and ultrastructural cytochemical methods, i. e. by means of LTA antibodies labelled with ferritin, or concanavalin A labelled with ferritin or colloidal gold. All these methods proved the LTA to be located on the outer cell surface of most group A streptococcus strains. The differences in the intensity of labelling paralelled the hydrophobicity of the strains, being substantially higher in the strains exhibiting a high degree of hydrophobicity. Treatment of streptococci with pronase or trypsin led to a complete loss of surface-located LTA. On the other hand, pepsin treatment of streptococci under mild conditions resulted in an increased amount of surface-located LTA in some strains. On the isolated cell walls, LTA could be demonstrated only on the outer surface of the walls. These findings correlated well with the presumed role of group A streptococcus LTA in the adherence of streptococci to the epithelial cells which is accomplished with the aid of surface-located LTA molecules. |
| Databáze: | OpenAIRE |
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