Molecular characterization of cobia (Rachycentron canadum) CD4 homologues revealed the first evidence of soluble CD4 in fish
| Autor: | Hung Bao Tran, Yen-Hung Lee, Hso-Chi Chaung, Putri Nurhanida Rizky, Stanley Rob McDaniel Padgett, Ta-Chih Cheng |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Fish Proteins Structural similarity Aquatic Science Biology 03 medical and health sciences Complementary DNA Extracellular Environmental Chemistry Animals RNA Messenger chemistry.chemical_classification Cobia Messenger RNA Phylogenetic tree Gene Expression Regulation Developmental 04 agricultural and veterinary sciences General Medicine biology.organism_classification Amino acid Perciformes Transmembrane domain 030104 developmental biology chemistry Biochemistry CD4 Antigens 040102 fisheries 0401 agriculture forestry and fisheries |
| Zdroj: | Fishshellfish immunology. 99 |
| ISSN: | 1095-9947 |
| Popis: | The co-receptor CD4 plays an important role in distinguishing between helper T-cell (Th) and cytotoxic T lymphocyte (CTL). In the present study, we investigated the molecular features of CD4-2 cDNA to facilitate understanding of their roles in cobia (Rachycentron canadum). Two CD4-2 molecules have been identified and exhibited 16.10% amino acids identity with each other. The cDNA of CD4-2A consists of a 993 bp ORF encoding 330 aa with long intracytoplasmic tail containing conserved protein tyrosine kinase p56Lck binding (C-X-C) motif, a transmembrane region, and two extracellular Ig-like (Ig-like) domains are predicted. Comparatively, the cDNA of cobia CD4-2B consists of a 990 bp ORF encoding 329 aa without a transmembrane domain as well as C-X-C motif, and three Ig-like domains are present. Homology comparison showed that the CD4-2A aa sequence of cobia showed high similarity and similar structural features to CD4-2 from other species, while the deduced CD4-2B protein shares higher structural similarity to CD4-1 group. Phylogenetic analysis indicated that cobia CD4-2A was closer with CD4-2 molecules in other fish species, distant from the clade formed by fish CD4-1 and mammalian CD4 sequences. However, cobia CD4-2B grouped with other known teleost CD4-1 sequences. The expression pattern of CD4-2A and CD4-2B mRNA during the embryonic development followed the trend of an initial increase after fertilized, providing evidence of maternal transfer of CD4-2 homologues to the developing cobia embryos and larvae. All of these results are useful for better understanding of cell-mediated immunity of cobia. |
| Databáze: | OpenAIRE |
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