SLC26A11 (KBAT) in Purkinje Cells Is Critical for Inhibitory Transmission and Contributes to Locomotor Coordination
| Autor: | Chris I. De Zeeuw, Manoocher Soleimani, Martijn Schonewille, María Fernanda Vinueza Veloz, Sharon Barone, Jie Xu, Freek E. Hoebeek, Negah Rahmati, Nahuel Rodolfo Ben Hamida |
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| Přispěvatelé: | Netherlands Institute for Neuroscience (NIN) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0303 health sciences
Cerebellum General Neuroscience Purkinje cell General Medicine Neurotransmission Biology Inhibitory postsynaptic potential gamma-Aminobutyric acid humanities Cell biology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure nervous system Chloride channel medicine Premovement neuronal activity Reversal potential Neuroscience 030217 neurology & neurosurgery 030304 developmental biology medicine.drug |
| Zdroj: | eNeuro, 3(3):e0028-16.2016. Society for Neuroscience |
| ISSN: | 2373-2822 |
| DOI: | 10.1523/eneuro.0028-16.2016 |
| Popis: | Chloride homeostasis determines the impact of inhibitory synaptic transmission and thereby mediates the excitability of neurons. Even though cerebellar Purkinje cells (PCs) receive a pronounced inhibitory GABAergic input from stellate and basket cells, the role of chloride homeostasis in these neurons is largely unknown. Here we studied at both the cellular and systems physiological level the function of a recently discovered chloride channel, SLC26A11 or kidney brain anion transporter (KBAT), which is prominently expressed in PCs. Using perforated patch clamp recordings of PCs, we found that a lack of KBAT channel in PC-specific KBAT KO mice (L7-KBAT KOs) induces a negative shift in the reversal potential of chloride as reflected in the GABAA-receptor-evoked currents, indicating a decrease in intracellular chloride concentration. Surprisingly, bothin vitroandin vivoPCs in L7-KBAT KOs showed a significantly increased action potential firing frequency of simple spikes, which correlated with impaired motor performance on the Erasmus Ladder. Our findings support an important role for SLC26A11 in moderating chloride homeostasis and neuronal activity in the cerebellum. |
| Databáze: | OpenAIRE |
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