A Contribution to Identification of Novel Regulators of Plant Response to Sulfur Deficiency: Characteristics of a Tobacco Gene UP9C, Its Protein Product and the Effects of UP9C Silencing
| Autor: | Jolanta Łukomska, Frantz Liszewska, Katarzyna Zientara, Małgorzata Lewandowska, Marta Piecho, Agnieszka Sirko, Anna Wawrzyńska, Grzegorz Moniuszko, Igor Zhukov, Paweł Hodurek, Victoria J. Nikiforova |
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| Rok vydání: | 2010 |
| Předmět: |
Sulfate assimilation
sulfur deficit Molecular Sequence Data Arabidopsis Plant Science Biology tobacco Transcriptome transcription regulators Gene Expression Regulation Plant Gene expression Metabolome Gene silencing Amino Acid Sequence Gene Silencing Gene Molecular Biology Research Articles Plant Proteins Regulation of gene expression Genetics Sequence Homology Amino Acid biology.organism_classification Plants Genetically Modified gene expression Sulfur |
| Zdroj: | Molecular Plant |
| ISSN: | 1674-2052 |
| DOI: | 10.1093/mp/ssq007 |
| Popis: | Extensive changes in plant transcriptome and metabolome have been observed by numerous research groups after transferring plants from optimal conditions to sulfur (S) deficiency. Despite intensive studies and recent important achievements, like identification of SLIM1/EIL3 as a major transcriptional regulator of the response to S-deficiency, many questions concerning other elements of the regulatory network remain unanswered. Investigations of genes with expression regulated by S-deficiency stress encoding proteins of unknown function might help to clarify these problems. This study is focused on the UP9C gene and the UP9-like family in tobacco. Homologs of these genes exist in other plant species, including a family of four genes of unknown function in Arabidopsis thaliana (LSU1-4), of which two were reported as strongly induced by S-deficit and to a lesser extent by salt stress and nitrate limitation. Conservation of the predicted structural features, such as coiled coil region or nuclear localization signal, suggests that these proteins might have important functions possibly mediated by interactions with other proteins. Analysis of transgenic tobacco plants with silenced expression of UP9-like genes strongly argues for their significant role in regulation of plant response to S-deficit. Although our study shows that the UP9-like proteins are important components of such response and they might be also required during other stresses, their molecular functions remain a mystery. |
| Databáze: | OpenAIRE |
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