Inflammatory profile of apical periodontitis associated with liver fibrosis in rats: histological and immunohistochemical analysis
| Autor: | Carlos Estrela, Gustavo Sivieri-Araujo, Tiago Novaes Pinheiro, Cristiane Cantiga-Silva, C. B. M. Cardoso, Luciano Tavares Angelo Cintra, Juan J. Segura-Egea, Edilson Ervolino, P H C de Oliveira, J P Azevedo |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal de Goiás (UFG), University of Sevilla, Dental School of Amazonas State University |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Liver Cirrhosis
Male Pathology medicine.medical_specialty Necrosis 0206 medical engineering Inflammation 02 engineering and technology Bone resorption 03 medical and health sciences 0302 clinical medicine Fibrosis oral infection medicine Animals Rats Wistar General Dentistry liver fibrosis Periodontitis Tumor Necrosis Factor-alpha business.industry Interleukin 030206 dentistry apical periodontitis medicine.disease 020601 biomedical engineering cytokines Rats Staining inflammation Cytokines Immunohistochemistry medicine.symptom business Periapical Periodontitis |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2021-06-25T10:57:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Aim: To investigate the effects of liver fibrosis (LF) on the pro-inflammatory mediators and periapical bone resorption of apical periodontitis (AP) in rats. Methodology: Forty male Wistar rats were distributed into four groups: C – control, AP – rats with AP, LF – rats with LF, AP + LF – rats with AP and LF. LF was induced by carbon tetrachloride administration for 8 weeks and surgical bile duct ligation for 4 weeks; AP was induced in the teeth of rats by dental pulp exposure to the oral environment for 30 days. Jaws and livers were removed after euthanasia. Haematoxylin and Eosin (H&E) and Picrosirius Red (PSR) staining were used to confirm fibrosis in the livers. The jaws were analysed using H&E staining, immunohistochemical assays of interleukin (IL)-1β, IL-6 and tumour necrosis factor-alpha (TNF-α). Student’s t-test and Mann–Whitney’s U-test were used for statistical analysis (P |
| Databáze: | OpenAIRE |
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