Comprehensive tropane alkaloids analysis and retrospective screening of contaminants in honey samples using liquid chromatography-high resolution mass spectrometry (Orbitrap)
| Autor: | Antonia Garrido Frenich, José Luis Martínez Vidal, Roberto Romero-González, Ana Romera-Torres |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
030309 nutrition & dietetics
Formic acid chemistry.chemical_element Food Contamination Orbitrap Mass Spectrometry law.invention 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology law Retrospective Studies 0303 health sciences Chromatography Magnesium Extraction (chemistry) Tropane 04 agricultural and veterinary sciences Honey Contamination 040401 food science Solvent chemistry Methanol Food Science Chromatography Liquid Tropanes |
| Zdroj: | Food research international (Ottawa, Ont.). 133 |
| ISSN: | 1873-7145 |
| Popis: | A wide-scope analytical method was developed and validated for the determination of tropane alkaloids (TAs) in honey samples. A simple and fast extraction procedure, using a mixture of methanol/water/formic acid (75/25/0.4, v/v/v) as extraction solvent, followed by a clean-up with graphitized black carbon (GBC) and magnesium sulphate was optimized, and compounds were analysed by liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS-Orbitrap). Validation of the proposed method provided adequate linearity (R2 > 0.99), trueness (recoveries 71–120%) and precision (relative standard deviation, RSD ≤ 20.1%), with limits of quantitation (LOQs) at 20 µg/kg (except anisodamine and scopolamine at 40 µg/kg) and a significant matrix effect (≤−50%). Nineteen honey samples were analysed, but only one was positive, containing 27 µg/kg of scopolamine. Additionally, a post-targeted screening was performed, and 47% of samples were contaminated with different herbicides, insecticides and veterinary drugs. Therefore, the proposed analytical method is a powerful tool for both targeted TAs and post-targeted contaminant analyses. |
| Databáze: | OpenAIRE |
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