Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (Sparus aurata L.)
| Autor: | María Ángeles Esteban, Cristóbal Espinosa-Ruíz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Antioxidant QH301-705.5 medicine.medical_treatment Glutathione reductase QH426-470 Aquatic Science Andrology 03 medical and health sciences antioxidant enzymes skin regeneration Gene expression Genetics medicine Biology (General) Ecology Evolution Behavior and Systematics chemistry.chemical_classification Skin repair fish 030102 biochemistry & molecular biology Ecology biology integumentary system gilthead seabream (Sparus aurata L.) Mucus skin healing Hsp70 030104 developmental biology Enzyme chemistry Catalase biology.protein mucosal immunity |
| Zdroj: | Fishes Volume 6 Issue 2 Fishes, Vol 6, Iss 15, p 15 (2021) |
| ISSN: | 2410-3888 |
| DOI: | 10.3390/fishes6020015 |
| Popis: | This study investigated the antioxidant enzyme activities in the skin mucus of gilthead seabream (Sparus aurata L.) at 3 and 7 days post-wounding (dpw). The expression levels of the genes that encode stress proteins (grp170, grp94, grp75, sod and hsp70) and skin regeneration-related proteins (tf, igf1, tgfb1, der1, apo1 and erdj-3) in the skin also were determined. Mucus and skin samples were obtained from the left and right flanks of non-wounded and wounded fish. In both flanks of the wounded fish, catalase and glutathione reductase activities in the skin mucus increased (p < 0.05) at 3 and 7 dpw (100 ± 31% and 111 ± 25%, respectively), whereas superoxide dismutase activity increased (p < 0.05) only at 7 dpw (135 ± 15%). The expression levels of stress proteins in the skin of the wounded flank of the wounded fish mainly increased at 7 dpw (grp170 increased to 288 ± 85%, grp94 to 502 ± 143%, grp75 to 274 ± 69%, sod to 569 ± 99%, and hsp70 increased to 537 ± 14%) (p < 0.05). However, the expression levels of the tissue regeneration-related genes varied depending on the flank investigated, on the experimental time, and on the gene studied. To the best of our knowledge, this is the first work to determine the effect of a wound in different skin parts of the same fish. |
| Databáze: | OpenAIRE |
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