Aflatoxin B1 acute effects on three hepatic urea cycle enzymes using semiautomated methods: a model for Reye's syndrome
| Autor: | Paul M. Newberne, Peter M. Thurlow, Robert K. Desai, Henry Brown |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Male
medicine.medical_specialty Ornithine transcarbamylase Carbamoyl-Phosphate Synthase (Ammonia) Carbamyl Phosphate Toxicology chemistry.chemical_compound Ornithine Carbamoyltransferase Aflatoxins Internal medicine medicine Citrulline Reye's syndrome Animals Urea Reye Syndrome Pharmacology Arginase medicine.disease Rats Disease Models Animal Endocrinology chemistry Biochemistry Liver Colorimetry |
| Zdroj: | Toxicology and applied pharmacology. 53(2) |
| ISSN: | 0041-008X |
| Popis: | Urea cycle enzyme activities may be determined through the colorimetric analysis of urea and citrulline, which are intermediates in the cycle. A new semiautomated method was used to measure the activities of carbamyl phosphate synthetase, ornithine transcarbamylase, and arginase in liver samples from male Fischer rats (120–150 g) given a toxic dose of aflatoxin B 1 . Carbamyl phosphate synthetase and ornithine transcarbamylase activities were 61 and 67%, respectively, of control values, 24 hr after a 3 mg/kg dose. Arginase activity was within the normal range. This pattern of ureas cycle enzyme activities is similar to that reported in biopsy specimens from children with Reye's syndrome. |
| Databáze: | OpenAIRE |
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