Pheophorbide a from Capsosiphon fulvescens Inhibits Advanced Glycation End Products Mediated Endothelial Dysfunction
| Autor: | Dongho Lee, Jin Won Lee, Kwon Woo Park, Cheong Tae Kim, Kwang Won Lee, Ji Sun Oh, Chung Oui Hong, Mi Hyun Nam |
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| Rok vydání: | 2015 |
| Předmět: |
Chlorophyll
Glycation End Products Advanced Pharmaceutical Science Biology Monocytes Analytical Chemistry Proinflammatory cytokine chemistry.chemical_compound Chlorophyta Glycation Drug Discovery Cell Adhesion Human Umbilical Vein Endothelial Cells medicine Humans Endothelial dysfunction Inflammation Pharmacology chemistry.chemical_classification Reactive oxygen species Monocyte Organic Chemistry Endothelial Cells Atherosclerosis medicine.disease Endothelial stem cell medicine.anatomical_structure Complementary and alternative medicine chemistry Biochemistry Pheophorbide A Cytokines Molecular Medicine Advanced glycation end-product Reactive Oxygen Species |
| Zdroj: | Planta Medica. 82:46-57 |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/s-0035-1557829 |
| Popis: | During hyperglycemia, the first step toward the formation of advanced glycation end products is the nonenzymatic glycation between the carbonyl group of a sugar and the primary amino group of a protein. Advanced glycation end products are then produced through more complex reactions. Reactive oxygen species derived from advanced glycation end products may play a key role in inflammation of the endothelium, leading to the complications seen in diabetes. Glycolaldehyde-induced advanced glycation end products have been reported to express proinflammatory cytokines, such as tumor necrosis factor-α and interleukin-1β. This study focused on Capsosiphon fulvescens, a Capsosiphonaceae type of green algae that has shown potential as a functional food material. Pheophorbide a, an anti-glycation compound, was isolated from C. fulvescens by extraction using a mixture of ethanol and water, followed by column fractionation of the resulting extract. The compound separated from C. fulvescens was identified by means of high-performance liquid chromatography combined with mass spectrometry. Pheophorbide a showed scavenging activity of the intracellular reactive oxygen species as well as monocyte adhesiveness inhibitory activity on the human myelomonocytic cell line (THP-1) and human umbilical vein endothelial cells cocultivation system. The mRNA levels of inflammation-related genes such as monocyte chemoattractant protein-1 and interleukin-6 were significantly decreased by pheophorbide a, and advanced glycation end products-stimulated tumor necrosis factor-α and interleukin-1β were downregulated as well. These results indicate that pheophorbide a has significant reactive oxygen species-scavenging activity, monocyte adhesive inhibitory activity, and downregulatory activity of cytokines related to inflammation affecting the endothelium. Pheophorbide a could therefore be a promising candidate for modulating endothelial cell dysfunction. |
| Databáze: | OpenAIRE |
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