Molecular prevalence of bovine noroviruses and neboviruses detected in central-eastern Tunisia
| Autor: | Pierre Pothier, Jérôme Kaplon, Khira Sdiri-Loulizi, Zaydoun Aouni, Katia Ambert-Balay, Mouna Hassine-Zaafrane, Mahjoub Aouni |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Tunisia Genotype Sequence analysis animal diseases Molecular Sequence Data Cattle Diseases Biology Feces fluids and secretions Medical microbiology Virology Genetic variation medicine Animals Phylogeny Caliciviridae Infections Strain (biology) Norovirus Calicivirus virus diseases Genetic Variation General Medicine Sequence Analysis DNA RNA-Dependent RNA Polymerase Gastroenteritis Neboviruses Herd Capsid Proteins Cattle Caliciviridae Sequence Alignment |
| Zdroj: | Archives of virology. 157(8) |
| ISSN: | 1432-8798 |
| Popis: | Two genetically distinct bovine enteric caliciviruses are known: noroviruses of genogroup III (NoVsGIII), which are genetically related to human noroviruses, and neboviruses, which represent a new calicivirus genus. To investigate the presence of NoVsGIII and nebovirus strains in diarrheic calves in Tunisia, a total of 169 faecal specimens were collected from January 2006 to October 2010 from different cattle herds located in the central-east regions. RT-PCRs and sequencing were carried out using primers targeting the 3' end of the polymerase gene of NoVsGIII and neboviruses. This study revealed that NoVsGIII and nebovirus are endemic in diarrheic calves in Tunisia. NoVsGIII infections, all with genotype 2, had an apparent molecular prevalence of 16.6 % and were more frequent than nebovirus infections. NoVsGIII infections showed clear seasonality, with a peak in winter. Nebovirus infections, with a prevalence of 3.0 %, were all related to the reference strain Bo/Nebraska/80/US. |
| Databáze: | OpenAIRE |
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