Inositol 1,4,5-trisphosphate receptor type 3 plays a protective role in hepatocytes during hepatic ischemia-reperfusion injury
| Autor: | Antônio Carlos Melo Lima Filho, Dabny Goulart Missiaggia, Meenakshisundaram Ananthanarayanan, Mateus T. Guerra, Fernanda O. Lemos, Roberta Cristelli Fonseca, Cristiano Xavier Lima, André G. Oliveira, M. Fatima Leite, Matheus de Castro Fonseca, Michael H. Nathanson, Marcone Loiola dos Santos, Rodrigo M. Florentino, Andressa França, Paula Vieira Teixeira Vidigal |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Necrosis Physiology medicine.medical_treatment Liver transplantation Protective Agents Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Humans Inositol 1 4 5-Trisphosphate Receptors Inositol Calcium Signaling Promoter Regions Genetic Molecular Biology Mice Knockout NFATC Transcription Factors Chemistry NFAT Cell Biology Inositol trisphosphate receptor medicine.disease DNA Demethylation Mice Inbred C57BL Transplantation Disease Models Animal 030104 developmental biology Endocrinology Liver Reperfusion Injury Hepatocytes medicine.symptom Reperfusion injury Hepatic Thrombosis 030217 neurology & neurosurgery |
| Zdroj: | Cell Calcium |
| ISSN: | 0143-4160 |
| Popis: | Hepatic ischemia-reperfusion injury is seen in a variety of clinical conditions, including hepatic thrombosis, systemic hypotension, and liver transplantation. Calcium (Ca(2+)) signaling mediates several pathophysiological processes in the liver, but it is not known whether and how intracellular Ca(2+) channels are involved in the hepatocellular events secondary to ischemia-reperfusion. Using an animal model of hepatic ischemia-reperfusion injury, we observed a progressive increase in expression of the type 3 isoform of the inositol trisphosphate receptor (ITPR3), an intracellular Ca(2+) channel that is not normally expressed in healthy hepatocytes. ITPR3 expression was upregulated, at least in part, by a combination of demethylation of the ITPR3 promoter region and the increased transcriptional activity of the nuclear factor of activated T-cells (NFAT). Additionally, expression of pro-inflammatory interleukins and necrotic surface area were less pronounced in livers of control animals compared to liver-specific ITPR3 KO mice subjected to hepatic damage. Corroborating these findings, ITPR3 expression and activation of NFAT were observed in hepatocytes of liver biopsies from patients who underwent liver ischemia caused by thrombosis after organ transplant. Together, these results are consistent with the idea that ITPR3 expression in hepatocytes plays a protective role during hepatic injury induced by ischemia-reperfusion. |
| Databáze: | OpenAIRE |
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