Genome wide binding (ChIP-Seq) of murine Bapx1 and Sox9 proteins in vivo and in vitro
| Autor: | Shyam Prabhakar, Thomas Lufkin, Vibhor Kumar, V. Sivakamasundari, Petra Kraus, Sook Peng Yap, Sumantra Chatterjee |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell type lcsh:QH426-470 Mouse Biology Biochemistry Genome ChIP-Seq 03 medical and health sciences 0302 clinical medicine In vivo Data in Brief Genetics Microarray analysis techniques Ligand binding assay Wild type Molecular biology In vitro Cell biology lcsh:Genetics 030104 developmental biology Occupancy profiling by NGS Molecular Medicine Homeobox Genome binding 030217 neurology & neurosurgery Bapx1 Biotechnology |
| Zdroj: | Genomics Data, Vol 10, Iss C, Pp 51-53 (2016) Genomics Data |
| ISSN: | 2213-5960 |
| Popis: | This work pertains to GEO submission GSE36672, in vivo and in vitro genome wide binding (ChIP-Seq) of Bapx1/Nkx3.2 and Sox9 proteins. We have previously shown that data from a genome wide binding assay combined with transcriptional profiling is an insightful means to divulge the mechanisms directing cell type specification and the generation of tissues and subsequent organs [1]. Our earlier work identified the role of the DNA-binding homeodomain containing protein Bapx1/Nkx3.2 in midgestation murine embryos. Microarray analysis of EGFP-tagged cells (both wildtype and null) was integrated using ChIP-Seq analysis of Bapx1/Nkx3.2 and Sox9 DNA-binding proteins in living tissue. |
| Databáze: | OpenAIRE |
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