Characterization of Streptomyces MITKK-103, a newly isolated actinomycin X2-producer
| Autor: | Kazuhiko Kurosawa, Chokyun Rha, T. G. Sambandan, Anthony J. Sinskey, V. P. Bui, J. L. VanEssendelft, Ion Ghiviriga, Philip A. Lessard, Laura B. Willis |
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| Rok vydání: | 2005 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data HL-60 Cells Streptomyces galbus Applied Microbiology and Biotechnology Streptomyces DNA Ribosomal Microbiology Agar plate RNA Ribosomal 16S Sequence Homology Nucleic Acid Humans Ribosomal DNA Phylogeny Soil Microbiology biology Strain (chemistry) Streptomycetaceae Nucleic Acid Hybridization General Medicine Pigments Biological Sequence Analysis DNA Ribosomal RNA biology.organism_classification Bacterial Typing Techniques Dactinomycin Microscopy Electron Scanning Actinomycetales Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 72(1) |
| ISSN: | 0175-7598 |
| Popis: | A new actinomycete strain designated MITKK-103 was isolated from the soil of a flowerpot using a humic acid agar medium. The newly isolated strain was able to produce a large amount of actinomycin X2 even under nonoptimized growing conditions and serves as a promising source of this antibiotic. Actinomycin X2 has higher cytotoxicity toward cultured human leukemia (HL-60) cells than does actinomycin D, and it induces cell death via apoptosis. A nearly complete 16S ribosomal DNA (rDNA) sequence from the isolate was determined and found to have high identity (98.5–100%) with Streptomyces galbus, Streptomyces griseofuscus, and Streptomyces padanus, indicating that MITKK-103 belongs to the genus Streptomyces. The isolate clustered with species belonging to the S. padanus clade in a 16S-rDNA-based phylogenetic tree and showed 75% overall homology to S. padanus ATCC 25646 in DNA–DNA relatedness analysis. Although the growth of the isolate was somewhat different from the three species mentioned, the strain MITKK-103 most closely resembles S. padanus on the basis of the morphological and phenotypic characteristics, phylogenetic analysis, and genotypic data. As such, this is the first report of a strain of S. padanus capable of producing actinomycins. |
| Databáze: | OpenAIRE |
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