Effect of antibodies on chemiluminescence and on killing of Streptococcus sobrinus by polymorphonuclear leukocytes
| Autor: | J. J. Soet, J. Graaff, C. J. Bosch-Tijhof, M. Raamsdonk |
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| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
Blood Bactericidal Activity medicine.drug_class Neutrophils Immunology Granulocyte Dental Caries Monoclonal antibody Microbiology Streptococcus sobrinus Neutrophil Activation law.invention Phagocytosis law medicine Humans General Dentistry Chemiluminescence Antiserum biology Immune Sera Antibodies Monoclonal biology.organism_classification Streptococcaceae Antibodies Bacterial stomatognathic diseases medicine.anatomical_structure Polyclonal antibodies Luminescent Measurements biology.protein Antibody |
| Zdroj: | Oral microbiology and immunology. 11(4) |
| ISSN: | 0902-0055 |
| Popis: | The effect of a polyclonal antiserum and OMVU10, a monoclonal antibody reactive with Antigen B of Streptococcus sobrinus, on the interaction of polymorphonuclear leukocytes with S. sobrinus was studied, using chemiluminescence and bacterial killing assays. Increased stimulation of neutrophils as measured in the chemiluminescence assays was established when S. sobrinus was preincubated with polyclonal antiserum or when polyclonal antiserum was added to the reaction mixture. Higher counts were measured in comparison to preimmune serum. After 90 min, 52% of S. sobrinus preincubated with polyclonal antiserum was killed. Killing was also increased when polyclonal antiserum was added to the reaction mixture in comparison to the controls. No killing was found when bacteria were preincubated with OMVU10 or when OMVU10 was added to the reaction mixture in comparison to Clone 24, a control antibody. |
| Databáze: | OpenAIRE |
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