LncRNA TUG1 attenuates ischaemia-reperfusion-induced apoptosis of renal tubular epithelial cells by sponging miR-144-3p via targeting Nrf2
| Autor: | Yuan Ruan, Fan Cheng, Siqi Li, Wu Chen, Weimin Yu, Yucheng Qi, Wei Li, Ting Rao, Cong Liu, Sheng Zhao, Xiangjun Zhou |
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| Rok vydání: | 2021 |
| Předmět: |
Male
NF-E2-Related Factor 2 Ischemia Apoptosis medicine.disease_cause Nrf2 Cell Line Gene Knockout Techniques Mice Downregulation and upregulation microRNA medicine Animals Kidney LncRNA TUG1 business.industry Gene Expression Profiling Epithelial Cells Cell Biology Original Articles Hypoxia (medical) medicine.disease Endoplasmic Reticulum Stress In vitro Mitochondria Disease Models Animal MicroRNAs Oxidative Stress medicine.anatomical_structure Kidney Tubules Gene Expression Regulation Reperfusion Injury renal ischaemia/reperfusion injury Cancer research miR‐144‐3p Molecular Medicine Kidney Diseases RNA Interference RNA Long Noncoding Original Article Disease Susceptibility medicine.symptom business Reactive Oxygen Species Oxidative stress Biomarkers Signal Transduction |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 |
| Popis: | Renal ischaemia/reperfusion (I/R) injury may induce kidney damage and dysfunction, in which oxidative stress and apoptosis play important roles. Long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) are reported to be closely related to renal I/R, but the specific molecular mechanism is still unclear. The purpose of this research was to explore the regulatory effect of lncRNA TUG1 on oxidative stress and apoptosis in renal I/R injury. This research revealed that in renal I/R injury and hypoxia/reperfusion (H/R) injury in vitro, the expression level of lncRNA TUG1 was upregulated, and oxidative stress levels and apoptosis levels were negatively correlated with the expression level of lncRNA TUG1. Using bioinformatics databases such as TargetScan and microRNA.org, microRNA‐144‐3p (miR‐144‐3p) was predicted to be involved in the association between lncRNA TUG1 and Nrf2. This study confirmed that the level of miR‐144‐3p was significantly reduced following renal I/R injury and H/R injury in vitro, and miR‐144‐3p was determined to target Nrf2 and inhibit its expression. In addition, lncRNA TUG1 can reduce the inhibitory effect of miR‐144‐3p on Nrf2 by sponging miR‐144‐3p. In summary, our research shows that lncRNA TUG1 regulates oxidative stress and apoptosis during renal I/R injury through the miR‐144‐3p/Nrf2 axis, which may be a new treatment target for renal I/R injury. |
| Databáze: | OpenAIRE |
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